Basic research to overcome resistance to anti-mitotic drugs

我们的目的是确定参与抗癌药物延长有丝分裂阻滞后细胞死亡的分子。当我们鉴定出的一种新分子CAMP被耗尽的细胞用微管抑制剂处理时，我们发现有丝分裂停止后细胞死亡加速，特别是在癌细胞系中。肿瘤细胞株CAMP的表达有高于正常细胞株的趋势，表明癌细胞的生存依赖于CAMP。抗凋亡的Bcl-2家族成员Mcl-1和Bcl-xL的表达在CAMP缺失的细胞中下降，提示CAMP可以作为抗癌治疗的靶点。

We aimed to identify molecules involved in cell death after prolonged mitotic arrest by anti-cancer drugs. When cells depleted of CAMP, a novel molecule we identified, were treated with microtubule inhibitors, we found the acceleration of cell death after mitotic arrest specifically in cancer cell lines. There was a tendency that cancer cell lines showed higher expression of CAMP than normal cell lines, implying that cancer cell survival is dependent on CAMP. Expression of Mcl-1 and Bcl-xL, anti-apoptotic Bcl-2 family members, was decreased in CAMP-depleted cells, suggesting that CAMP can be a target for anti-cancer therapy.