Study on ultra-precision viscosity measurement using a fluorescent probe

点击翻译按钮获取中文摘要

In this study, we suggest a novel method for evaluating the viscosity of small samples based on the rotational diffusion coefficient of the nano probe by using fluorescence polarization method. In the case of using the fluorescence probe with lifetime less than 10 ns ( i.e. Fluorescein, fluorescence lifetime 4.3ns, diameter 1.1nm) , it is possible to accelerate the rate of fluorescence polarization analysis for viscosity measurement to 100MHz. Furthermore, by using the probe with diameter of 1nm, the measurement accuracy of the viscosity using a diffusion model of the Brownian motion is improved due to minimizing the influence of the inertial force of probe.As a result, we construct the fundamental system to evaluate the rotational diffusion coefficient for small sample. We verify the feasibility of proposed method for evaluating the viscosity of small sample based on the rotational diffusion coefficient.