Development of a high-pressure freeze trap method of protein crystals

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X-ray radiation damage is a serious problem for the high-pressure protein crystallography, because the method uses a diamond anvil cell and it is necessary to perform experiments at room temperature. In this study, we have tried to develop a new method that makes protein crystals frozen under high-pressure condition. A new apparatus has been developed. However, we found the mother liquid oozes from the crystal when we pressurize protein crystals, and that degrades crystal quality during the freezing process. We could not find a workaround for this problem.