Determination of atherogenic properties in plasmalogens including trans-fatty acid.

纤溶酶原是甘油磷脂的一个亚类，具有自由基敏感的化学结构，被认为是一种内源性抗动脉粥样硬化因子。反式脂肪酸是公认的致动脉粥样硬化的膳食成分，但其作用机制尚不完全清楚。我们发现，反式脂肪酸喂养大鼠4周后，反式脂肪酸被丰富地结合到血浆蛋白原分子中。将反式脂肪酸喂养的大鼠的部分低密度脂蛋白应用于培养的血管内皮细胞，可增强单核细胞与血管内皮细胞的黏附因子VCAM1和单核细胞趋化因子MCP1的基因和蛋白表达。这些结果表明，反式脂肪酸结合在纤溶酶原中是血管内致动脉粥样硬化变化的诱因。

Plasmalogen is a subclass of glycerophospholipids with radical-sensitive chemical structure, and suggested as an endogenous anti-atherogenic factor. Fatty acids with trans form are well-known atherogenic dietary components, however, the mechanisms are not full clarified. We found that a trans-form FA was abundantly incorporated into plasmalogen molecules by feeding of trans fatty acids for 4 weeks in rats. Application of a LDL fraction of trans fatty acid-fed rats to a cultured blood vessel endothelial cell, HUVEC, enhanced gene and protein expression of VCAM1, a adhesion factor for monocyte to blood vessel endothelium, and MCP1, a chemoattractant for monocyte in the cells. These results suggest that trans fatty acids incorporated into plasmalogen is an inducer of the atherogenic changes in the blood vessels.