Quantitative targeted absolute proteomics-based diagnostics for personalized cancer chemotherapy

基于绝对蛋白质组学的个性化癌症化疗的定量靶向诊断

• 批准号: 24659063
• 负责人: TERASAKI TETSUYA
• 金额: $ 2.5万
• 依托单位: Tohoku University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Challenging Exploratory Research
• 财政年份: 2012
• 资助国家: 日本
• 起止时间: 2012-04-01 至 2014-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-24659063/
• 关键词: 分子標的薬; 定量プロテオミクス; リン酸化; 増殖因子受容体; 個別化医療; がん; 標的絶対定量プロテオミクス; 個別化; 脳腫瘍; 受容体

There has been increasing demands for molecular-targeted drugs-based personalized cancer chemotherapy. Our findings provide the basis that the characteristics of human malignant brain tumors can be stratified according to the absolute protein expression levels of target receptors. Simultaneous and highly sensitive quantitative targeted phospho-proteomics has also been established.

基于分子靶向药物的个性化癌症化疗的需求日益增加。我们的研究结果提供的基础上，人类恶性脑肿瘤的特点，可以分层根据靶受体的绝对蛋白表达水平。同时，高灵敏度的定量靶向磷酸蛋白质组学也已建立。

项目成果

Quantitative Expression of Human Drug Transporter Proteins in Lung Tissues : Analysis of Regional, Gender and Inter-individual differences by Liquid Chromatography-Tandem Mass Spectrometry. E-pub ahead of print

肺组织中人类药物转运蛋白的定量表达：通过液相色谱-串联质谱分析区域、性别和个体间差异。

• 发表时间: 2013
• 期刊: J Pharm Sci
• 作者: Sakamoto A;Matsumaru T;Yamamura N;Uchida Y;Tachikawa M;Ohtsuki S;Terasaki T
• 通讯作者: Terasaki T

Blood-brain barrier (BBB) pharmacoproteomics : a new research field opened up by quantitative targeted absolute proteomics (QTAP). Drug Delivery to the Brain–Physiological Concepts, Methodologies and Approaches, Hammarlund-Udenaes M, de Lange E, and Thorn

血脑屏障（BBB）药物蛋白质组学：定量靶向绝对蛋白质组学（QTAP）开辟的新研究领域——脑部药物递送——生理学概念、方法论和途径，Hammalund-Udenaes M、de Lange E和Thorn。

• 发表时间: 2014
• 作者: Uchida Y;Tachikawa M;Ohtsuki S;Terasaki T
• 通讯作者: Terasaki T

血液脳関門—白血球細胞遊走に関わる接着分子の標的定量プロテオミクス解析

血脑屏障——参与白细胞迁移的粘附分子的靶向定量蛋白质组学分析

• 发表时间: 2014
• 作者: 佐藤和貴;立川正憲;内田康雄;大槻純男;寺崎哲也
• 通讯作者: 寺崎哲也

Identification of transporters associated with Etoposide sensitivity of stomach cancer cell lines and methotrexate sensitivity of breast cancer cell lines by quantitative targeted absolute proteomics

通过定量靶向绝对蛋白质组学鉴定与胃癌细胞系依托泊苷敏感性和乳腺癌细胞系甲氨蝶呤敏感性相关的转运蛋白

• DOI: 10.1124/mol.112.081083
• 发表时间: 2013
• 期刊: Mol Pharmacol
• 影响因子: 3.6
• 作者: Obuchi W;Ohtsuki S;Uchida Y;Ohmine K;Yamori T;Terasaki T
• 通讯作者: Terasaki T

A Study Protocol for Quantitative Targeted Absolute Proteomics (QTAP) by LC-MS/MS : Application for Inter-Strain Differences in Protein Expression Levels of Transporters, Receptors, Claudin-5, and Marker Proteins at the Blood-Brain Barrier in ddY, FVB, an

通过 LC-MS/MS 进行定量靶向绝对蛋白质组学 (QTAP) 的研究方案：在 ddY、FVB 中血脑屏障转运蛋白、受体、Claudin-5 和标记蛋白的蛋白表达水平中的菌株间差异的应用

• 发表时间: 2013
• 期刊: Fluids and Barriers of the CNS
• 影响因子: 7.3
• 作者: Uchida Y;Tachikawa M;Obuchi W;Hoshi Y;Tomioka Y;Ohtsuki S;Terasaki T
• 通讯作者: Terasaki T