Influence of mechanical stress applied to ES/iPS cells on organelle control and cell metabolism/differentiation

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Our objective was to establish a method for inducing the differentiation of renal tubular cells from human ES cells, and to clarify the significance of mechanical stress in the process of differentiation. KSP was used as a marker for renal tubular cell differentiation. Differentiation to an intermediate mesoderm was promoted using GSK3β inhibitor. Epithelization was then promoted using a reduced serum medium containing EGF, which successfully yielded approximately 5% KSP-positive cells. We then investigated the influence of mechanical stress on cell differentiation using HKC-8, a human proximal tubular cell, under different conditions. However, it was not possible to apply sufficient mechanical stress. We are therefore planning to conduct further investigations in the future.