Influence of mechanical stress applied to ES/iPS cells on organelle control and cell metabolism/differentiation

ES/iPS 细胞机械应力对细胞器控制和细胞代谢/分化的影响

• 批准号: 24659454
• 负责人: ITOH Hiroshi
• 金额: $ 2.41万
• 依托单位: Keio University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Challenging Exploratory Research
• 财政年份: 2012
• 资助国家: 日本
• 起止时间: 2012 至 无数据
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-24659454/
• 关键词: ES細胞; 細胞分化; 腎臓細胞; メカニカルストレス

Our objective was to establish a method for inducing the differentiation of renal tubular cells from human ES cells, and to clarify the significance of mechanical stress in the process of differentiation. KSP was used as a marker for renal tubular cell differentiation. Differentiation to an intermediate mesoderm was promoted using GSK3β inhibitor. Epithelization was then promoted using a reduced serum medium containing EGF, which successfully yielded approximately 5% KSP-positive cells. We then investigated the influence of mechanical stress on cell differentiation using HKC-8, a human proximal tubular cell, under different conditions. However, it was not possible to apply sufficient mechanical stress. We are therefore planning to conduct further investigations in the future.

本研究旨在建立人ES细胞向肾小管细胞分化的诱导方法，并探讨机械应力在分化过程中的作用。KSP被用作肾小管细胞分化的标志物。使用GSK 3 β抑制剂促进向中间中胚层的分化。然后使用含有EGF的减少血清培养基促进上皮化，其成功地产生约5%的KSP阳性细胞。然后，我们研究了机械应力对细胞分化的影响，使用HKC-8，人近端肾小管细胞，在不同的条件下。然而，不可能施加足够的机械应力。因此，我们计划在未来进行进一步调查。

项目成果

ヒトES細胞を用いた尿細管上皮細胞への分化誘導の試み

尝试使用人ES细胞诱导分化为肾小管上皮细胞

• 发表时间: 2013
• 作者: 中津祐介;岩下未咲;松永泰花;福嶋俊明;迫田秀之;櫛山暁史;鎌田英明;内田隆史;浅野知一郎;Suganami T;山口慎太郎
• 通讯作者: 山口慎太郎

ヒトES細胞を用いた尿細管上皮細胞の分化誘導の試み

尝试使用人ES细胞诱导肾小管上皮细胞分化

• 发表时间: 2013
• 作者: 山口慎太郎;本間康一郎;門川俊明;森実隆司;鈴木さゆり;藤井静花;脇野修;林晃一;伊藤裕
• 通讯作者: 伊藤裕