Induction of keratinocyte stem cell based on epidermal transcription factors

PTTG1是参与控制细胞生长、分化和凋亡的转录因子。本研究旨在研究PTTG1在气液界面三维培养中角质形成细胞增殖和分化及形成分层表皮样结构中的作用。PTTG1在未分化的正常角质形成细胞中特异性表达，并在分化过程中下调。在空气暴露诱导的分化过程中，PTTG1在角质形成细胞中的组成性过表达导致了未分化的表型，即层状层增厚，分化标记物表达受到抑制，细胞周期调节蛋白在S期和G2/M期激活，增殖活性保持不变。这些发现提示PTTG1可能在维持角质形成细胞的未分化表型中发挥重要作用。

PTTG1 is the transcription factor involved in the control of cell growth, differentiation, and apoptosis. The present study was performed to examine the role of PTTG1 in proliferation and differentiation of keratinocytes with formation of a stratified epidermoid structure in air-liquid interface 3-dimensional culture. PTTG1 was expressed specifically in undifferentiated normal keratinocytes and downregulated during differentiation. The constitutive overexpression of PTTG1 in keratinocytes during air exposure-induced differentiation resulted in an undifferentiated phenotype, i.e., thickening of the stratified layers, suppression of differentiation marker expression, and retention of proliferative activity with activation of cell cycle regulatory proteins in the S and G2/M phases. These findings suggested that PTTG1 may play an important role in maintenance of the undifferentiated phenotype of keratinocytes.