Analyses of transcriptional factor Fra-1 function with a focus on EMT

以 EMT 为重点的转录因子 Fra-1 功能分析

• 批准号: 24659583
• 负责人: HOSHINO Isamu
• 金额: $ 2万
• 依托单位: Chiba University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Challenging Exploratory Research
• 财政年份: 2012
• 资助国家: 日本
• 起止时间: 2012-04-01 至 2014-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-24659583/
• 关键词: Fra-1; 食道扁平上皮癌; HMGA1; HMMR; EMT; 上皮間葉移行; 足場非依存性増殖

We have reported that Fra-1 was correlated with invasion and metastasis in esophageal squamous cell carcinoma(ESCC). Herein, we tried to clarify the mechanism of the Fra-1 expression in ESCC. We constructed Fra-1 knock down cells using siRNA in Fra-1 high expressed cells, TE10 and TE11. Fra-1 knock down induced reducing of cell proliferation, migration, and invasion potentials. Then, we explored the Fra-1 regulated genes using microarray and they were analyzed by BIOBASE Upstream Analysis. HMGA1 was a candidate which was regulated by Fra-1 and HMMR was detected as a gene which was regulated by HMGA1. Moreover, immunohistochemcal staining using clinical ESCC samples showed that HMMR was a significant prognostic factor in ESCC patients.

我们已经报道了Fra-1与食管鳞状细胞癌（ESCC）的侵袭和转移有关。本研究试图阐明Fra-1在食管鳞癌中的表达机制。我们在Fra-1高表达细胞TE 10和TE 11中使用siRNA构建Fra-1敲低细胞。Fra-1基因敲低可导致细胞增殖、迁移和侵袭能力降低。利用基因芯片技术检测Fra-1调控基因，并采用BIOBASE上游分析技术进行分析。HMGA 1是受Fra-1调控的候选基因，HMMR是受HMGA 1调控的基因。此外，使用临床ESCC样本的免疫组化染色显示HMMR是ESCC患者的重要预后因素。

项目成果

食道扁平上皮癌におけるFra-1制御遺伝子の網羅的検索

食管鳞癌中Fra-1调控基因的综合研究

• 发表时间: 2013
• 作者: 豊住武司、星野敢、阿久津泰典;ほか
• 通讯作者: ほか

食道扁平上皮癌におけるFra-1 制御遺伝子の網羅的検索

食管鳞癌中Fra-1调控基因的综合研究

• 发表时间: 2013
• 作者: 豊住 武司;星野 敢;阿久津 泰典;羽成 直行;森 幹人;西森 孝典;村上 健太郎;赤沼 直毅;遠藤 悟史;磯崎 由佳;竹下 修由;丸山 哲朗;小松 亜紀;松原 久裕
• 通讯作者: 松原 久裕