Cancer stem cell for peritoneal metastasis in abdominal cavity

我们发现CD90+CD45-的人腹膜游离细胞亚群具有间充质干细胞的特性，并在TGFb刺激下分化为肌成纤维细胞，与MKN45联合注射可显著提高裸鼠腹膜转移形成率。组织学检查发现，在转移结节内种植了大量的MSC，且主要位于纤维区。酪氨酸激酶抑制剂达沙替尼在体外对MSC的增殖有明显的抑制作用，但对MKN45的增殖无明显影响。然而，口服达沙替尼显著抑制了MKN45腹膜转移的发展，并减少了转移结节的纤维形成。流式细胞仪检测CD326+肿瘤细胞率可作为判断腹膜转移严重程度及化疗疗效的可靠生物标志物。

We found that a minor subset of human intraperitoneal free cells with CD90+CD45- displayed the characteristics of mesenchymal stem cell, and differentiated into myofibroblast by the stimulation with TGFb, and intraperitoneal coinjection of with MKN45 significantly enhanced the rate of metastatic formation in the peritoneum of nude mice. Histological examination revealed that many MSC were engrafted in metastatic nodules and were mainly located at the fibrous area. Dasatinib, a tyrosine kinase inhibitor, strongly inhibited the proliferation of MSC but not MKN45 in vitro. Nevertheless, oral administration of Dasatinib significantly inhibited the development of peritoneal metastasis of MKN45, and resulted in reduced fibrillar formation of metastatic nodules. The rate of CD326+ tumor cells measured with FACS could be a reliable diagnostic biomarker to determine the severity of peritoneal metastasis as well as effectiveness of chemotherapy.