Development of artificial niche matrices for cardiac differentiation of stem cells

开发用于干细胞心脏分化的人工生态位基质

• 批准号: 24659597
• 负责人: NAKATANI Takeshi
• 金额: $ 2.41万
• 依托单位: National Cardiovascular Center Research Institute
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Challenging Exploratory Research
• 财政年份: 2012
• 资助国家: 日本
• 起止时间: 2012-04-01 至 2014-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-24659597/
• 关键词: 人工臓器学; 心筋梗塞; 移植・再生医療; 人工臓器

Stem cell-based myocardial regeneration has been emerged as alternative strategy for the heart transplantation or destination therapy using left ventricular assist system (LVAS). Especially self-beating cardiomyocytes are promising cells, but effective protocols to prepare the mature cardiomyocytes from various stem cells have not been clarified yet. The purpose of this article is find out the effect of stem cell culture niche (ECM proteins and substrate elasticities) on the cardiac differentiation, beating induction, and beating duration separately by using iPS cells (iPSs) and neonatal cardiomyocytes (NCMs). The cardiac differentiation step was greatly enhanced on Col-I-gel but the beating-induction step (a-MHC, TnC1, TnT2 expression) was effective on FN-culture dishes. The beating-duration step, which is assessed by counting the number of beating colonies of NCMs, preferred Col-I–gel. We also found the effective surface for keeping the undifferentiated stages of iPS cells.

基于干细胞的心肌再生已成为心脏移植或左心室辅助系统（LVAS）治疗的替代策略。特别是自跳动心肌细胞是一种很有发展前途的细胞，但从各种干细胞制备成熟心肌细胞的有效方法尚未明确。本研究旨在通过诱导多能干细胞（iPS）和新生儿心肌细胞（ncm）分别探讨干细胞培养生态位（ECM蛋白和底物弹性）对心脏分化、搏动诱导和搏动持续时间的影响。col - 1凝胶可显著增强心脏分化步骤，但在fn培养液上可有效增强心跳诱导步骤（a-MHC、TnC1、TnT2的表达）。通过计算ncm的击打菌落数量来评估的击打时间步骤，首选coli - gel。我们还发现了维持iPS细胞未分化阶段的有效表面。

项目成果

Paracorporeal ventricular assist device as a bridge to transplant candidacy in the era of implantable continuous-flow ventricular assist device

体外心室辅助装置是植入式连续流心室辅助装置时代移植候选者的桥梁

• DOI: 10.1007/s10047-013-0731-3
• 发表时间: 2013
• 期刊: J Artif Organs
• 影响因子: 1.3
• 作者: Iwashima Y;Yanase M;Horio T;Seguchi O;Murata Y;Fujita T;Toda K;Kawano Y;Nakatani T;Suwa H
• 通讯作者: Suwa H

PLA-PEGマルチブロックコポリマーの直接注入による心筋梗塞治療の検討

PLA-PEG多嵌段共聚物直接注射治疗心肌梗死的研究

• 作者: Fujita T;Toda K;Yanase M;Seguchi O;Murata Y;Ishibashi-Ueda H;Kobayashi J and Nakatani T;C.A. Agudelo;Carlos A Agudelo;染川将太;染川将太
• 通讯作者: 染川将太

心不全診療 Q&A エキスパート106人からの回答

106位专家心力衰竭治疗问答解答

• 发表时间: 2012
• 作者: Fujita T;Toda K;Yanase M;Seguchi O;Murata Y;Ishibashi-Ueda H;Kobayashi J and Nakatani T;C.A. Agudelo;Carlos A Agudelo;染川将太;染川将太;Naoki KOBAYASHI;染川将太;四倉寛子;Sato T;Tetsuji Yamaoka;平田みつひ;池田貴之;中谷武嗣;中谷武嗣;中谷武嗣;中谷武嗣;中谷武嗣;中谷武嗣
• 通讯作者: 中谷武嗣

Off-pump coronary artery bypass grafting for a left main lesion due to cardiac llograt vasculopathy in Japan: first report of a case

日本因心脏血管病变而进行非体外循环冠状动脉旁路移植术治疗左主干病变：首例病例报告

• DOI: 10.1007/s00595-013-0651-0
• 发表时间: 2014
• 期刊: Surg Today
• 影响因子: 2.5
• 作者: Fujita T;Kobayashi J;Hata H;Murata Y;Seguchi O;Yanase M;Shimahara Y;Sato S;Nakatani T
• 通讯作者: Nakatani T

Magnetic resonance imaging detection of an uncommon granuloma formation after endothelial progenitor cells transplantation

磁共振成像检测内皮祖细胞移植后罕见的肉芽肿形成

• DOI: 10.3233/bme-130768
• 发表时间: 2013
• 期刊: Bio-Medical Materials and Engineering
• 影响因子: 1
• 作者: 中谷武嗣;秦広樹;藤田知之;小林順二郎;村田欣洋;瀬口理;簗瀬正伸;堀由美子;和田恭一;植田初江;宮田茂樹;内藤博昭;中谷武嗣;中谷武嗣;C. A. Agudelo
• 通讯作者: C. A. Agudelo