Designing protein-incorporating scaffolds for periodontal tissue engineering

近年来，间充质干细胞和生物可降解支架在牙周组织再生中的应用受到广泛关注。目前最重要的挑战之一是加速新骨组织的形成。在这项研究中，我们筛选了各种生长因子及其组合，以确定最佳的因子，以结合到支架中促进骨形成。结果发现，在骨组织支架材料的制备中，BMP-2的掺入是最有效的。进一步研究鉴定与生物可降解聚合物支架有亲和力的多肽。结果发现，具有特定序列的多肽与聚乳酸具有亲和力。根据这一结果，我们认为，当与生长因子融合时，该多肽将有效地构建生长因子/支架复合材料。

Recently much attention has been paid to periodontal tissue regeneration using mesenchymal stem cells and biodegradable scaffolds. One of the most important challenges at this moment is to accelerate the formation of new bone tissues. In this study, we screened various growth factors and their combinations to identify the best factors to be incorporated into scaffolds for the enhanced bone formation. As a result, we found that incorporation of BMP-2 into scaffold materials is most effective for the preparing bone tissue scaffolds. Further study was carried out to identify peptides that have an affinity for biodegradable polymer scaffolds. As a result, it was found that the peptide having a specific sequence exhibited an affinity for poly(lactic acid). According to this result, we propose that, when fused with a growth factor, this peptide would be effective for constructing growth factor/scaffold composites.