Elucidation of structural basis for molecular recognition and supramolecular complex formation of intracellular lectins involved in post-endoplasmic reticulum quality control

阐明参与内质网后质量控制的细胞内凝集素的分子识别和超分子复合物形成的结构基础

• 批准号: 21370050
• 负责人: KATO Koichi
• 金额: $ 11.48万
• 依托单位: National Institutes of Natural Sciences Okazaki Research Facilities
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21370050/
• 关键词: 品質管理; 糖鎖; レクチン; NMR; 結晶構造解析

In cells, the carbohydrate moieties serve as tags for the quality control of glycoproteins through interactions with a variety of lectins. To elucidate the mechanisms underlying the glycoprotein quality control by the lectins that cycle between the endoplasmic reticulum and the Golgi, we provided the structural basis for their molecular recognition and complex formation by structural biological approaches such as X-ray crystallography. Furthermore, we successfully developed novel NMR methods for detailed analyses of conformational dynamics of the carbohydrate chains involved in this system.

在细胞中，碳水化合物部分通过与多种凝集素的相互作用来充当糖蛋白质量控制的标签。为了阐明由内质网和高尔基体之间循环的凝集素控制糖蛋白质量控制的机制，我们通过结构生物学方法（例如X射线晶体学）为它们的分子识别和复杂形成提供了结构性基础。此外，我们成功地开发了新型的NMR方法，用于详细分析该系统中涉及的碳水化合物链的构象动力学。

项目成果

Application of Paramagnetic NMR-Validated Molecular Dynamics Simulation to the Analysis of a Conformational Ensemble of a Branched Oligosaccharide

• DOI: 10.3390/molecules17066658
• 发表时间: 2012-06-01
• 期刊: MOLECULES
• 影响因子: 4.6
• 作者: Zhang, Ying;Yamamoto, Sayoko;Kato, Koichi
• 通讯作者: Kato, Koichi

Analysis of N-glycans in embryonated chicken egg chorioallantoic and amniotic cells responsible for binding and adaptation of human and avian influenza viruses

• DOI: 10.1007/s10719-008-9193-x
• 发表时间: 2009-05-01
• 期刊: GLYCOCONJUGATE JOURNAL
• 影响因子: 3
• 作者: Sriwilaijaroen, Nongluk;Kondo, Sachiko;Suzuki, Yasuo
• 通讯作者: Suzuki, Yasuo

Application of paramagnetic NMR to analyses of conformations and dynamics of oligosaccharides

顺磁核磁共振在寡糖构象和动力学分析中的应用

• 发表时间: 2011
• 作者: S.Yamamoto;T.Yamaguchi;Y.Zhang;M.Erdelyi;C.Griesinger;K.Kato
• 通讯作者: K.Kato

Structural views of functional and pathological roies of sugar chains

糖链功能性和病理性的结构观点

• 发表时间: 2011
• 作者: Sahlan;M.;Zako;T.;Tai;P. T.;Ohtaki;A.;Noguchi;K.;Maeda;M.;Miyatake;H.;Dohmae;N.;Yohda;M.;K.Kato
• 通讯作者: K.Kato

Introduction of a paramagnetic tag for NMR conformational analysis of oligosacc harides

引入用于寡糖 NMR 构象分析的顺磁标签

• 发表时间: 2009
• 作者: T.Yamaguchi;M.Yamamoto;S.Yamamoto;H.Yagi;M.Erdelyi;C.Griesinger;K.Kato
• 通讯作者: K.Kato