Reconstruction of the normal vasculature for the strategy of new cancer therapy.

我们假设，重建肿瘤中的正常血管可能导致缺氧条件的改善，从而有助于抗癌药物递送的潜在改善。将肿瘤细胞注射到SCID小鼠的背侧皮下组织中。2周后，给荷瘤小鼠腹腔注射顺铂，以使肿瘤和肿瘤血管第一步消退。两周后，将人EC或人ES衍生的EC移植到肿瘤的坏死区域以诱导正常血管生成用于第二步。显微镜下观察发现移植的内皮细胞与宿主小鼠血管形成了内皮细胞膜。由EC重建的血管表现出肿瘤血管系统的正常化，导致肿瘤体积的显著减小。因此，该模型是未来通过构建可靠的药物递送系统和改善缺氧条件来开发新的癌症疗法的有用模型。

We hypothesized that reconstruction of normal blood vessels in tumors may lead to improvement of hypoxic conditions, contributing to potential improvements in the delivery of anticancer drugs. Tumor cells were injected into the dorsal subcutis of SCID mice. After 2 weeks, the tumor-bearing mice were injected cisplatin intraperitoneally to regress tumors and tumor vessels for the first step. Two weeks later, human ECs or human ES-derived ECs were transplanted into necrotic regions of the tumor to induce normal angiogenesis for the second step. Microscopic observations revealed that the transplanted ECs formed anastomoses with the host mouse vasculature. Blood vessels reconstituted by ECs exhibited the normalization of tumor vasculature, resulting in a significant reduction in the tumor volume. Thus, this model is a useful model for the future development of new cancer therapies by the construction of reliable drug delivery systems and the improvement of hypoxic conditions.