Reconstruction of the normal vasculature for the strategy of new cancer therapy.

重建正常脉管系统以制定新的癌症治疗策略。

• 批准号: 24659904
• 负责人: EZAKI Taichi
• 金额: $ 1.83万
• 依托单位: Tokyo Women's Medical University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Challenging Exploratory Research
• 财政年份: 2012
• 资助国家: 日本
• 起止时间: 2012-04-01 至 2014-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-24659904/
• 关键词: 腫瘍血管; 血管正常化; 血管再構築; 再生医療; Normalization; ドラッグデリバリーシステム; ES細胞; scaffold; 血管新生

We hypothesized that reconstruction of normal blood vessels in tumors may lead to improvement of hypoxic conditions, contributing to potential improvements in the delivery of anticancer drugs. Tumor cells were injected into the dorsal subcutis of SCID mice. After 2 weeks, the tumor-bearing mice were injected cisplatin intraperitoneally to regress tumors and tumor vessels for the first step. Two weeks later, human ECs or human ES-derived ECs were transplanted into necrotic regions of the tumor to induce normal angiogenesis for the second step. Microscopic observations revealed that the transplanted ECs formed anastomoses with the host mouse vasculature. Blood vessels reconstituted by ECs exhibited the normalization of tumor vasculature, resulting in a significant reduction in the tumor volume. Thus, this model is a useful model for the future development of new cancer therapies by the construction of reliable drug delivery systems and the improvement of hypoxic conditions.

我们假设，重建肿瘤中的正常血管可能导致缺氧条件的改善，从而有助于抗癌药物递送的潜在改善。将肿瘤细胞注射到SCID小鼠的背侧皮下组织中。2周后，给荷瘤小鼠腹腔注射顺铂，以使肿瘤和肿瘤血管第一步消退。两周后，将人EC或人ES衍生的EC移植到肿瘤的坏死区域以诱导正常血管生成用于第二步。显微镜下观察发现移植的内皮细胞与宿主小鼠血管形成了内皮细胞膜。由EC重建的血管表现出肿瘤血管系统的正常化，导致肿瘤体积的显著减小。因此，该模型是未来通过构建可靠的药物递送系统和改善缺氧条件来开发新的癌症疗法的有用模型。

项目成果

Vasohibin-2の抑制が消化管における自然発症腫瘍に影響を及ぼすか

Vasohibin-2 的抑制是否会影响胃肠道自发性肿瘤？

• 发表时间: 2013
• 作者: 北原秀治;鈴木康弘;森島正恵;吉井明日香;松居彩;佐藤靖史;江崎太一
• 通讯作者: 江崎太一

臨床的放射線耐性腫瘍を用いた新たな癌治療モデルの確立

利用临床抗辐射肿瘤建立新的癌症治疗模式

• 发表时间: 2012
• 作者: 北原秀治;鈴木康弘;森島正恵;吉井明日香;松居彩;佐藤靖史;江崎太一;北原秀治;北原秀治;北原秀治
• 通讯作者: 北原秀治

Transplanting normal endothelial cells into tumor mass retards the tumor growth by vascular reconstruction and vascular normalization

将正常内皮细胞移植到肿瘤块中通过血管重建和血管正常化延缓肿瘤生长

• 发表时间: 2013
• 作者: Shuji Kitahara;Miwako Nishio;Shunichi Morikawa;Kazuko Nakada;Asuka Yoshii;Kumiko Saeki;Taichi Ezaki
• 通讯作者: Taichi Ezaki

Establishment of a normalized revascularization mouse model using tumor transplantation

肿瘤移植标准化血运重建小鼠模型的建立

• DOI: 10.1016/j.ajoms.2013.02.004
• 发表时间: 2013
• 期刊: Journal of Oral and Maxillofacial Surgery, Medicine, and Pathology
• 作者: Shimizu K;Asakura M;Shinga J;Sato Y;Kitaha Hoshino K;Kaisho T;Schoenberger SP;Ezaki T;Fujii S;Yumiko Momokia
• 通讯作者: Yumiko Momokia

Establishment of a normalized revascularization mouse model using tumor transplantation. Journal of Oral and Maxillofacial Surgery, Medicine, and Pathology

利用肿瘤移植建立标准化血运重建小鼠模型。

• 发表时间: 2014
• 期刊: Journal of Oral and Maxillofacial Surgery, Medicine, and Pathology
• 作者: Momoki Y;Kitahara S;Kuwahara K;Sasaki R;Ando T
• 通讯作者: Ando T