A morphological and functional study on the development and regeneration of lymphatic vessels.

淋巴管发育和再生的形态和功能研究。

• 批准号: 05670018
• 负责人: EZAKI Taichi
• 金额: $ 1.34万
• 依托单位: Kumamoto University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1993
• 资助国家: 日本
• 起止时间: 1993 至 1994
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-05670018/
• 关键词: Microcirculation; Lymphatic vessel; Endothelial cell; Development; Lymphangiogenesis; Multiple immunostaining; Monoclonal antibody; Endothelial cell growth factor; 再生; 固体発生; 免疫組織化学

The development and regeneration of lymphatic vessels have been investigated using morphological and functional approaches. In order to identify lymphatic capillaries and proliferating cells in situ, a mouse monoclonal antibody against rat lymphatics (B27) and a technique for double immunostaining of proliferating cell nuclear antigen (PCNA) with other markers have been employed.(1) Development of rat lymphatics ; During fetal stages, lymphatics have been poorly developed. Within a half day after birth, large molecules such as carbon particles were taken up into central lacteals of the small intestine. After 24 hrs, however, no carbon particle was detected in the lymphatics and the morphological development and proliferation of lymphatics followed after the development of blood capillary networks.(2) Regeneration of rat lymphatics ; The ligation of the thoracic duct or removal of lymph nodes caused severe lymph edema and cell infiltration in the first few days, and then resulted in loc … More al adhesion after several days. The proliferation of lymphatic endothelial cells and the formation of newly formed lymphatics usually occurred after the local changes. However, B27-positive immature mesenchymal cells might play an initial role in the development of so called 'extravaskularen Saftbahnen' (Kihara, 1951) for absorption of tissue fluids during the early pathological stages.(3) Effect of growth factors on lymphatic endothelial cells ; Effects of epidermal cell growth factor (ECGF) and basic fibroblast growth factor (b-FGF) on lymphatic endothelial cells have been examined in vitro. Comparing with blood endothelial cells, the effects of both factors were not so great for the proliferation and morphological changes of lymphatic endothelial cells.These results suggest that lymphatics develop and regenerate supplementarily following the changes in blood vessels, but that the function of the lymphatics in terms of the absorption of tissue fluids, particularly at the early stages, may not reflect their immature appearance. Less

淋巴管的发育和再生已经使用形态学和功能的方法进行了研究。为了在原位鉴别毛细淋巴管和增殖细胞，采用了小鼠抗大鼠淋巴细胞单克隆抗体（B27）和增殖细胞核抗原（PCNA）与其他标记物的双重免疫染色技术。(1)大鼠视神经的发育;在胎儿阶段，视神经发育不良。在出生后的半天内，大分子如碳颗粒被吸收到小肠的中央乳糜管中。然而，24小时后，在血管中未检测到碳颗粒，并且血管的形态发育和增殖跟随在毛细血管网络的发育之后。(2)结扎胸导管或切除淋巴结后，在最初几天内引起严重的淋巴水肿和细胞浸润，然后导致意识丧失， ...更多信息 几天后的粘附力。淋巴管内皮细胞的增生和新生血管的形成通常发生在局部病变之后。然而，B27阳性的未成熟间充质细胞可能在所谓的“血管外基质Saftbahnen”（Kihara，1951）的发展中起初始作用，用于在早期病理阶段吸收组织液。(3)表皮细胞生长因子（ECGF）和碱性成纤维细胞生长因子（b-FGF）对淋巴管内皮细胞的作用已在体外研究。与血液内皮细胞相比，两种因素对淋巴管内皮细胞增殖和形态变化的影响均较小，提示淋巴管的发育和再生是随血管的变化而发生的，但淋巴管吸收组织液的功能，特别是早期的功能，可能并不反映其未成熟的外观。少

项目成果

Taichi Ezaki: "The identification of proliferating cell nuclear antigen(PCNA) on rat tissue cryosections and its application to double immunostaining with other markers." Arch.Histol.Cytol.,. (印刷中).

Taichi Ezaki：“大鼠组织冷冻切片上增殖细胞核抗原 (PCNA) 的鉴定及其在与其他标记物双重免疫染色中的应用。”（正在出版）。

Taichi Ezaki: "The identification of proliferating cell nuclesr antigen (PCNA) on rat tissue cryosections and its application to double immunostaining with other markers." Ar ch. Histol. Cytol., 印刷中. (1995)

Taichi Ezaki：“大鼠组织冷冻切片上增殖细胞核抗原 (PCNA) 的鉴定及其在与其他标记物双重免疫染色中的应用。”Ar ch。

Taichi Ezaki: "The identification of proliferating cell nuclear antigen (PCNA) on rat tissue cryosections and its application to double immunostaining with other markers." Arch.Histol.Cytol. (in press). (1995)

Taichi Ezaki：“大鼠组织冷冻切片上增殖细胞核抗原 (PCNA) 的鉴定及其在与其他标记物双重免疫染色中的应用。”