Mechanisms for the collection of cells by lymphatics

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The mechanisms for the collection of cells by lymphatic vessels have been investigated using morphological and functional approaches. In order to characterize lymphatics on molecular bases, a rat monoclonal antibody against mouse lymphatics (LA1O2) has been produced.1. Morphological and functional changes in lymphatics during local inflammation:(1) Establishment of a method for visualization of blood vessels by tomato-lection to distiguish from lymphatics. (2)Production of a monoclonal antibody (LA102) specifc to mouse lymphatic endothelial cells.2. 3-dimensional relationship between lymphoid cells and lymphatics during inflammation:(1)In organ transplantation models, inflammatory cells used sugar-lectin binding at the first recognition stage. (2) Their location to microvessels was studied by the confocal laser scan microscopy.3. Identification of molecules rich on and round lymphatic endothelial cells; (1) LA102 antigen is a27-30 kd noble protein expressed on lymphatics but not on blood vessels. (2) LA102 antigen has been under investigation in comparison with some extracellular matrices and chemokines.4. Functional characterization of LA102 molecule:LA102 antigen also exsist on the surface of some lymphoid cells, suggesting that this molecule may be involved in the cellular recognition between the cells and lymphatic endothelial cells. In situ binding assays on tissure cryosections are now under investigation to see the functional aspect of LA102 antigen.These results suggest that LA102. antigen may play an important role in the mechanisms for the collection of lymphoid cells by lymphatic vessels under both normal and pathological conditions.