Mechanisms for the collection of cells by lymphatics

淋巴管收集细胞的机制

• 批准号: 12670020
• 负责人: EZAKI Taichi
• 金额: $ 2.56万
• 依托单位: Tokyo Woman's Medical University (2001)Kumamoto University (2000)
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2000
• 资助国家: 日本
• 起止时间: 2000 至 2001
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-12670020/
• 关键词: Microcirculation; Lymphatic Vessel; Endothelial Cell; Monoclonal Antibody; Lymphocyte Recirculation; Cell Adhesion; Lectin Receptor; Lymhangiogenesis; モノクローナル抗体; リンパ球; 樹状細胞; リンパ管転移; レクチン様受容体; クッパー細胞; 肝臓; 糖鎖

The mechanisms for the collection of cells by lymphatic vessels have been investigated using morphological and functional approaches. In order to characterize lymphatics on molecular bases, a rat monoclonal antibody against mouse lymphatics (LA1O2) has been produced.1. Morphological and functional changes in lymphatics during local inflammation:(1) Establishment of a method for visualization of blood vessels by tomato-lection to distiguish from lymphatics. (2)Production of a monoclonal antibody (LA102) specifc to mouse lymphatic endothelial cells.2. 3-dimensional relationship between lymphoid cells and lymphatics during inflammation:(1)In organ transplantation models, inflammatory cells used sugar-lectin binding at the first recognition stage. (2) Their location to microvessels was studied by the confocal laser scan microscopy.3. Identification of molecules rich on and round lymphatic endothelial cells; (1) LA102 antigen is a27-30 kd noble protein expressed on lymphatics but not on blood vessels. (2) LA102 antigen has been under investigation in comparison with some extracellular matrices and chemokines.4. Functional characterization of LA102 molecule:LA102 antigen also exsist on the surface of some lymphoid cells, suggesting that this molecule may be involved in the cellular recognition between the cells and lymphatic endothelial cells. In situ binding assays on tissure cryosections are now under investigation to see the functional aspect of LA102 antigen.These results suggest that LA102. antigen may play an important role in the mechanisms for the collection of lymphoid cells by lymphatic vessels under both normal and pathological conditions.

通过淋巴管收集细胞的机制已经使用形态学和功能方法进行了研究。为了在分子基础上表征抗体，已经制备了抗小鼠抗体（LA 1 O2）的大鼠单克隆抗体。局部炎症时血管的形态和功能变化：（1）建立了一种鉴别血管的番茄凝集法。（2）抗小鼠淋巴管内皮细胞单克隆抗体（LA 102）的制备. 3-炎症过程中淋巴细胞与淋巴细胞之间的空间关系：（1）器官移植模型中，炎症细胞在第一识别阶段使用糖-凝集素结合。(2)共聚焦激光扫描显微镜观察其在微血管中的定位.淋巴管内皮细胞表面及周围分子的鉴定：（1）LA 102抗原是一种27 -30 kd的稀有蛋白，表达于血管内皮细胞，而不表达于血管。(2)LA 102抗原与一些细胞外基质和趋化因子进行了比较研究. LA 102分子的功能特点：LA 102抗原也存在于某些淋巴细胞表面，提示该分子可能参与淋巴细胞与淋巴管内皮细胞的细胞识别。组织冷冻切片上的原位结合试验正在研究中，以了解LA 102抗原的功能方面。抗原在正常和病理状态下淋巴管聚集淋巴样细胞的机制中可能起重要作用。

项目成果

Saiki T, Ezaki T, Ogawa M, Matsuno K: "Trafficking of host- and donor-derived dendritic cells in rat cardiac transplantation : Allosensitization in the spleen and hepatic nodes"Transplantation. 71(12). 1806-1815 (2001)

Saiki T、Ezaki T、Okawa M、Matsuno K：“大鼠心脏移植中宿主和供体来源的树突状细胞的贩运：脾和肝节点的同种异体致敏”移植。

Saiki T, Ezaki T, Ogawa M, Maeda K, Yagita H, Matsuno K: "In vivo roles of donor and host dendritic cells In allogeneic Immune response : cluster formation with host proliferating T cells"J Leukocyte Biol. 69(5). 705-712 (2001)

Saiki T、Ezaki T、Okawa M、Maeda K、Yagita H、Matsuno K：“供体和宿主树突状细胞在同种异体免疫反应中的体内作用：宿主增殖 T 细胞的簇形成”J 白细胞生物学。

Saiki T, Ezaki T, Ogawa M, Matsuno K,: "Trafficking of host- and donor-derived dendritic cells in rat cardiac transplantation: Allosensitization in the spleen and hepatic nodes."Transplantation. 71 (12). 1806-1815 (2001)

Saiki T、Ezaki T、Okawa M、Matsuno K，：“大鼠心脏移植中宿主和供体来源的树突状细胞的贩运：脾和肝节点的同种异体致敏。”移植。

Saiki T, Ezaki T, Ogawa M, Maeda K, Yagita H, Matsuno K,: "In vivo roles of donor and host dendritic cells in allogeneic immune response: cluster formation with host proliferating T cells."J Leukocyte Biol. 69 (5). 705-712 (2001)

Saiki T、Ezaki T、Okawa M、Maeda K、Yagita H、Matsuno K，：“供体和宿主树突状细胞在同种异体免疫反应中的体内作用：与宿主增殖 T 细胞形成簇。”J Leukcyto Biol。

Saiki T, Ezaki T, Ogawa M, Matsuno K: "Trafficking of host-and donor-derived dendritic cells in rat cardiac transplantation : Allosensitizatlon in the spleen and hepatic nodes"Transplantation. 71(12). 1806-1815 (2001)

Saiki T、Ezaki T、Okawa M、Matsuno K：“大鼠心脏移植中宿主和供体来源的树突状细胞的贩运：脾和肝节点的同种异体致敏”移植。