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Morphological and functional characterization of a specific marker for lymphatic vessels.

淋巴管特定标记物的形态和功能特征。

基本信息

批准号：
14207001
负责人：
EZAKI Taichi
金额：
$ 27.54万
依托单位：
Tokyo Women's Medical University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (A)
财政年份：
2002
资助国家：
日本
起止时间：
2002 至 2003
项目状态：
已结题

项目摘要

A specific marker for mouse lymphatic vessels has been characterized morphologically and functionally using a rat monoclonal antibody, LA102. 1.Specificity of LA102 antibody and tissue distribution of its antigen : (1)LA102 recognized mouse lymphatic vessels, but not blood vessels. (2)The antigen recognized by LA102 was localized on both luminal-and basal-sick cell membrane of lymphatic endothelium and was also highly condensed on the pinocytic or transport vesicle membrane. (3)LA102 positive endothelial cells were isolated from a benign lymphangioma and established as a cell line. 2.Immunobiological characterization of the LA102 antigen : (1)The isotype of LA102 antibody was rat IgG2b,κ. (2)The LA102 antigen was a glycoprotein with a molecular size of 25-27kDa. (3)LA102 antigen was also expressed on mainly T lymphocytes and cerebral tissues. 3.Molecular and genetical characterization of LA102 antigen : (1)LC-MASS analyzes revealed that LA102 antigen has a similar amino acid sequence to CD90 molecule. (2)Genetical analyzes of LA102 antigen have been underway comparing with CD90 and some other related molecules (such as cell adhesion molecules and chemokines). 4.Functional characterization of LA102 antigen : (1)Several experimental models for cell migration from the peritoneal cavity have been established. The diaphragm and omentum were the main lymphatic routes from the peritoneal cavity. (2)In an inflammatory model, LA102 antibody seemed to inhibit cell binding to various tissues, such as the liver, thymus, lymph nodes. These results suggest that LA102 might play an important role in the lymphoid cell migration and some transporting mechanisms through lymphatics under both normal and pathological conditions.

小鼠淋巴管的特异性标记物已经使用大鼠单克隆抗体LA 102在形态学和功能上表征。1. LA 102抗体的特异性及其抗原的组织分布：（1）LA 102识别小鼠淋巴管，不识别血管;（2）LA 102识别的抗原定位于淋巴管内皮细胞的腔病细胞膜和基底病细胞膜上，并高度浓缩于胞饮细胞膜或转运囊泡膜上。（3）从一良性淋巴管瘤组织中分离出LA 102阳性的内皮细胞，建立了细胞系。2. LA 102抗原的免疫生物学特性：（1）LA 102抗体的同种型为大鼠IgG 2b，κ。（2）LA 102抗原是一种糖蛋白，分子量为25- 27 kDa。（3）LA 102抗原主要表达于T淋巴细胞和脑组织。3. LA 102抗原的分子和遗传学特征：（1）LC-MASS分析表明LA 102抗原与CD 90分子具有相似的氨基酸序列。（2）LA 102抗原与CD 90及其他相关分子（如细胞粘附分子、趋化因子）的遗传学比较研究正在进行中。4. LA 102抗原的功能特性：（1）建立了几种细胞从腹腔迁移的实验模型。膈和网膜是腹膜腔的主要淋巴途径。(2)In在炎症模型中，LA 102抗体似乎抑制细胞与各种组织如肝、胸腺、淋巴结的结合。这些结果表明，在正常和病理条件下，LA 102可能在淋巴细胞迁移和通过淋巴管的一些转运机制中发挥重要作用。