Morphological and functional characterization of a specific marker for lymphatic vessels.

利用大鼠单克隆抗体LA102对小鼠淋巴管的特异性标记物进行了形态学和功能表征。1.LA102抗体的特异性及其抗原的组织分布：(1)LA102能识别小鼠淋巴管，但不能识别血管。(2) LA102所识别的抗原既定位于淋巴内皮的基底病细胞膜上，也高度凝聚于胞质或运输囊泡膜上。(3)从良性淋巴管瘤中分离LA102阳性内皮细胞并建立细胞系。2.LA102抗原的免疫生物学特性：(1)LA102抗体的同型为大鼠IgG2b、κ。(2) LA102抗原为糖蛋白，分子量为25 ~ 27kda。(3)LA102抗原也主要在T淋巴细胞和脑组织中表达。3.LA102抗原的分子和遗传特征：(1)LC-MASS分析显示LA102抗原与CD90分子具有相似的氨基酸序列。(2) LA102抗原已与CD90及其他相关分子（如细胞粘附分子、趋化因子）进行了遗传分析。4.LA102抗原的功能表征：(1)建立了几种细胞从腹腔迁移的实验模型。膈膜和网膜是腹膜腔的主要淋巴通道。(2)在炎症模型中，LA102抗体似乎可以抑制细胞与肝脏、胸腺、淋巴结等多种组织的结合。这些结果表明，在正常和病理条件下，LA102可能在淋巴样细胞的迁移和一些通过淋巴的运输机制中发挥重要作用。

A specific marker for mouse lymphatic vessels has been characterized morphologically and functionally using a rat monoclonal antibody, LA102. 1.Specificity of LA102 antibody and tissue distribution of its antigen : (1)LA102 recognized mouse lymphatic vessels, but not blood vessels. (2)The antigen recognized by LA102 was localized on both luminal-and basal-sick cell membrane of lymphatic endothelium and was also highly condensed on the pinocytic or transport vesicle membrane. (3)LA102 positive endothelial cells were isolated from a benign lymphangioma and established as a cell line. 2.Immunobiological characterization of the LA102 antigen : (1)The isotype of LA102 antibody was rat IgG2b,κ. (2)The LA102 antigen was a glycoprotein with a molecular size of 25-27kDa. (3)LA102 antigen was also expressed on mainly T lymphocytes and cerebral tissues. 3.Molecular and genetical characterization of LA102 antigen : (1)LC-MASS analyzes revealed that LA102 antigen has a similar amino acid sequence to CD90 molecule. (2)Genetical analyzes of LA102 antigen have been underway comparing with CD90 and some other related molecules (such as cell adhesion molecules and chemokines). 4.Functional characterization of LA102 antigen : (1)Several experimental models for cell migration from the peritoneal cavity have been established. The diaphragm and omentum were the main lymphatic routes from the peritoneal cavity. (2)In an inflammatory model, LA102 antibody seemed to inhibit cell binding to various tissues, such as the liver, thymus, lymph nodes. These results suggest that LA102 might play an important role in the lymphoid cell migration and some transporting mechanisms through lymphatics under both normal and pathological conditions.