A simple sequencing system of complete chloroplast genome using long PCR and NGS in coniferous species

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Two simple complete-sequencing systems of chloroplast genome were constructed for Coniferophyta species. One was the sequencing system using a next generation sequencer (NGS), and long PCRs designed with the arrangement information of the highly conserved regions among genera. The other was the system using the next generation sequencing of the geneme DNA extracted from crude chloroplast fraction of the leaf and bioinformatics. It could be possible to decode the chloroplast genome completely in a lot of coniferous species by using either of these two sequencing systems.