课题基金 / 基金详情

Genetic mapping in Japanese coniferous tree species using microsatellite DNA markers

Genetic mapping in Japanese coniferous tree species using microsatellite DNA markers
使用微卫星 DNA 标记对日本针叶树种进行遗传图谱分析
批准号:
13460070
负责人:
SHIRAISHI Susumu
金额:
$9.28万
依托单位:
依托单位国家:
日本
项目类别:
Grant-in-Aid for Scientific Research (B)
财政年份:
2001
资助国家:
日本
项目状态:
已结题
起止时间:
2001 至 2002

项目摘要

项目成果

SHIRAISHI Susumu的其他基金

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中文摘要
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英文摘要
To establish an efficient selection system of SSR (simple sequence repeats) DNA regions, TAC (triplex affinity capture) that is a new method instead of a molecular hybridization method, a modified PIMA (PCR-based isolation of microsatellite arrays) and so on was investigated. The results were as follows:1.Using model DNAs in which [CT]-repeats ([CT]_<10>,[CT]_<15>,[CT]_<20>, and [CT]_<25>) were inserted, an optimization of TAC was executed on pH and temperature conditions of the reaction. In pH 6.4 -7.0, highly repeated DNA fragments were selected efficiently.2.The original PIMA method was modified for the reliable screening of the recombinant colony in which SSR was inserted.3.The modified TAC and PIMA were applied into actual screening of SSRs from the genomes of Cryptomeria japonica, Pinus thunbergii, Chamaecyparis obtuse, and Acacia mangium. In C.japonica, About 300 SSR fragments were selected, and SCAR (sequence characterized amplified region) markers were designed based on their sequences.4.The materials of families for constructing linkage-maps were made in C.japonica (a haploid-endospermic family of Sawara-1), P.thunbergii(a haploid-endospermic family of Sima-64), and C.obtuse (a cross-pollinated family between Aira-32 and Satsuma-8).
期刊论文(6)
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会议论文
Nakashima, K., Shiraishi, S.: "Effective analysis of microsatellite in Chamaecyparis obtuse"Kyushu Forest Research. 56. 69-70 (2003)
Nakashima, K., Shiraishi, S.:“钝柏扁柏微卫星的有效分析”九州森林研究。
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通讯作者:
Widyatmoko, AYPBC., Shiraishi, H.: "Identification of Acacia auriculiformis and A. mangium using RAPD markers"Kyushu Journal of Forest Research. 54. 55-56 (2001)
Widyatmoko,AYPBC.,Shiraishi,H.:“使用 RAPD 标记鉴定金合欢和马占相思”九州森林研究杂志。
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通讯作者:
中島康介, 白石進: "ヒノキマイクロサテライト分析の効率化-Multiplex PCR化-"九州森林研究. 56. 69-70 (2003)
Kosuke Nakajima、Susumu Shiraishi:“提高柏树微卫星分析的效率 - 多重 PCR -” 九州森林研究 56. 69-70 (2003)。
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A simple sequencing system of complete chloroplast genome using long PCR and NGS in coniferous species
  • 批准号:
    25660124
  • 项目类别:
    Grant-in-Aid for Challenging Exploratory Research
  • 资助金额:
    $2.5万
  • 财政年份:
    2013
  • 负责人:
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  • 批准号:
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  • 项目类别:
    Grant-in-Aid for Challenging Exploratory Research
  • 资助金额:
    $2.5万
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    2012
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  • 批准号:
    22658050
  • 项目类别:
    Grant-in-Aid for Challenging Exploratory Research
  • 资助金额:
    $2.29万
  • 财政年份:
    2010
  • 负责人:
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Global transcriptome analysis of the pine wilt disease and application to the resistance breeding
  • 批准号:
    21380100
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
  • 资助金额:
    $11.23万
  • 财政年份:
    2009
  • 负责人:
    SHIRAISHI Susumu
  • 依托单位: