课题基金 / 基金详情

Genetic mapping in Japanese coniferous tree species using microsatellite DNA markers

Genetic mapping in Japanese coniferous tree species using microsatellite DNA markers
使用微卫星 DNA 标记对日本针叶树种进行遗传图谱分析
批准号:
13460070
负责人:
SHIRAISHI Susumu
金额:
$9.28万
依托单位:
依托单位国家:
日本
项目类别:
Grant-in-Aid for Scientific Research (B)
财政年份:
2001
资助国家:
日本
项目状态:
已结题
起止时间:
2001 至 2002

项目摘要

项目成果

SHIRAISHI Susumu的其他基金

相关文献

中文摘要
翻译
为建立高效的SSR(Simple Sequence Repeats)DNA区域选择体系,研究了取代分子杂交法的三重亲和捕获法(TAC)、改进的基于PCR的微卫星阵列分离(PIMA)等方法。结果如下:1.利用插入了[CT]-重复序列([CT]_<10>,[CT]_<15>,[CT]_<20>和[CT]_<25>)的模型DNA,对反应的pH和温度条件进行了TAC优化。在pH 6.4-7.0的条件下,对原有的PIMA方法进行了改进,使之能够可靠地筛选到插入SSR的重组菌落。3.将改进后的TAC和PIMA方法应用于柳杉、黑松、木通和马占相思基因组中SSR的实际筛选。本研究选择了300个左右的SSR片段,并根据其序列设计了SCAR标记。4.构建连锁图谱的家系材料分别来自日本锦鸡儿(Sawa-1的单倍体胚乳家系)、松柏(司马-的单倍体胚乳家系)和钝顶锦鸡儿(Aira-32与温州蜜柑的异花授粉家系)。
英文摘要
To establish an efficient selection system of SSR (simple sequence repeats) DNA regions, TAC (triplex affinity capture) that is a new method instead of a molecular hybridization method, a modified PIMA (PCR-based isolation of microsatellite arrays) and so on was investigated. The results were as follows:1.Using model DNAs in which [CT]-repeats ([CT]_<10>,[CT]_<15>,[CT]_<20>, and [CT]_<25>) were inserted, an optimization of TAC was executed on pH and temperature conditions of the reaction. In pH 6.4 -7.0, highly repeated DNA fragments were selected efficiently.2.The original PIMA method was modified for the reliable screening of the recombinant colony in which SSR was inserted.3.The modified TAC and PIMA were applied into actual screening of SSRs from the genomes of Cryptomeria japonica, Pinus thunbergii, Chamaecyparis obtuse, and Acacia mangium. In C.japonica, About 300 SSR fragments were selected, and SCAR (sequence characterized amplified region) markers were designed based on their sequences.4.The materials of families for constructing linkage-maps were made in C.japonica (a haploid-endospermic family of Sawara-1), P.thunbergii(a haploid-endospermic family of Sima-64), and C.obtuse (a cross-pollinated family between Aira-32 and Satsuma-8).
期刊论文(6)
专著(0)
科研奖励(0)
会议论文
Nakashima, K., Shiraishi, S.: "Effective analysis of microsatellite in Chamaecyparis obtuse"Kyushu Forest Research. 56. 69-70 (2003)
Nakashima, K., Shiraishi, S.:“钝柏扁柏微卫星的有效分析”九州森林研究。
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通讯作者:
Widyatmoko, AYPBC., Shiraishi, H.: "Identification of Acacia auriculiformis and A. mangium using RAPD markers"Kyushu Journal of Forest Research. 54. 55-56 (2001)
Widyatmoko,AYPBC.,Shiraishi,H.:“使用 RAPD 标记鉴定金合欢和马占相思”九州森林研究杂志。
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通讯作者:
中島康介, 白石進: "ヒノキマイクロサテライト分析の効率化-Multiplex PCR化-"九州森林研究. 56. 69-70 (2003)
Kosuke Nakajima、Susumu Shiraishi:“提高柏树微卫星分析的效率 - 多重 PCR -” 九州森林研究 56. 69-70 (2003)。
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通讯作者:
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  • 批准号:
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  • 资助金额:
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    2010
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  • 项目类别:
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  • 资助金额:
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