A simple system for developing the microsatellite markers using RSE-PCR and pyrosequencing

DNA分子标记是育种、遗传学和生态学研究的有力工具。在这些标记中，微卫星标记变异性大，含有大量的遗传信息。构建一种新的系统，引入新一代测序仪(焦磷酸测序仪)来获得基因组中巨大的微卫星区域序列。新系统显示了一种简单而廉价的微卫星开发的可能性。

DNA molecular markers were widely utilized as powerful tool in breeding, genetics, and ecology. Among these markers, microsatellite marker is highly variable, and possesses a large quantity of genetic information. Constructing a new system, a next generation sequencer (pyrosequencing) was introduced to gain the huge sequences of microsatellite regions in genomes. The new system showed the possibility for a simple and inexpensive microsatellite development.