A simple system for developing the microsatellite markers using RSE-PCR and pyrosequencing
A simple system for developing the microsatellite markers using RSE-PCR and pyrosequencing
批准号:
24658138
负责人:
SHIRAISHI Susumu
金额:
$2.5万
依托单位:
依托单位国家:
日本
项目类别:
Grant-in-Aid for Challenging Exploratory Research
财政年份:
2012
资助国家:
日本
项目状态:
已结题
起止时间:
2012 至 --
中文摘要
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英文摘要
DNA molecular markers were widely utilized as powerful tool in breeding, genetics, and ecology. Among these markers, microsatellite marker is highly variable, and possesses a large quantity of genetic information. Constructing a new system, a next generation sequencer (pyrosequencing) was introduced to gain the huge sequences of microsatellite regions in genomes. The new system showed the possibility for a simple and inexpensive microsatellite development.
期刊论文(4)
专著(0)
科研奖励(0)
会议论文
Development of tetranucleotide microsatellite markers in Pinus kesiya Royle ex Gordon
凯西亚松四核苷酸微卫星标记的开发
DOI:
10.1007/s12686-012-9814-3
发表时间:
2012
期刊:
Conservation Genetics Resources
影响因子:
1.1
作者:
[D. V. Thao, M. Yamashita, A. Watanabe, S. Shiraishi]
通讯作者:
S. Shiraishi
Isolation and characterization of tetranucleotide microsatellite markers for Pinus merkusii
梅库斯松四核苷酸微卫星标记的分离和表征
DOI:
--
发表时间:
2013
期刊:
Conservation Genet Resour
影响因子:
--
作者:
[Thao DV, Widyatmoko AYPBC, Guan L Watanabe A, Gotoh E, Shiraishi S]
通讯作者:
Shiraishi S
A simple sequencing system of complete chloroplast genome using long PCR and NGS in coniferous species
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批准号:25660124
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项目类别:Grant-in-Aid for Challenging Exploratory Research
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资助金额:$2.5万
-
财政年份:2013
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负责人:SHIRAISHI Susumu
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依托单位:
A new feed profiling using DNA information for wild-herbivores
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批准号:22658050
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项目类别:Grant-in-Aid for Challenging Exploratory Research
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资助金额:$2.29万
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财政年份:2010
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负责人:SHIRAISHI Susumu
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依托单位:
Global transcriptome analysis of the pine wilt disease and application to the resistance breeding
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批准号:21380100
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项目类别:Grant-in-Aid for Scientific Research (B)
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资助金额:$11.23万
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财政年份:2009
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负责人:SHIRAISHI Susumu
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依托单位:
Constructing a new breeding system for afforestation CDM in tropical Acacia forestry
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批准号:17208014
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项目类别:Grant-in-Aid for Scientific Research (A)
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资助金额:$29.12万
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财政年份:2005
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负责人:SHIRAISHI Susumu
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依托单位:
Genetic mapping in Japanese coniferous tree species using microsatellite DNA markers
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批准号:13460070
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项目类别:Grant-in-Aid for Scientific Research (B)
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资助金额:$9.28万
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财政年份:2001
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负责人:SHIRAISHI Susumu
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依托单位:
An evaluation system of resistance ability of Japanese black pine clones against pine wood nematode based on parentage diagnoses using DNA molecular markers
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批准号:12556024
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项目类别:Grant-in-Aid for Scientific Research (B)
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资助金额:$7.1万
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财政年份:2000
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负责人:SHIRAISHI Susumu
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依托单位:
Molecular systematical Studies of Cupressaceae and Taxodiaceae using DNA sequence information
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批准号:11460073
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项目类别:Grant-in-Aid for Scientific Research (B).
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资助金额:$9.28万
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财政年份:1999
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负责人:SHIRAISHI Susumu
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依托单位:
Construction of a new improvement system using DNA molecular technique in the forest tree species
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批准号:09556032
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项目类别:Grant-in-Aid for Scientific Research (B)
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资助金额:$8.45万
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财政年份:1997
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负责人:SHIRAISHI Susumu
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依托单位:
Molecular systematical Studies of conifer using DNA sequence information
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批准号:09460070
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项目类别:Grant-in-Aid for Scientific Research (B)
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资助金额:$7.42万
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财政年份:1997
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负责人:SHIRAISHI Susumu
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依托单位:
A new identification system of sugi (Cryptomeria japonica) cultivars based on DNA typing
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批准号:06556024
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项目类别:Grant-in-Aid for Scientific Research (A)
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资助金额:$6.14万
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财政年份:1994
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负责人:SHIRAISHI Susumu
-
依托单位:
海外基金