Global transcriptome analysis of the pine wilt disease and application to the resistance breeding

松材线虫病全转录组分析及其在抗性育种中的应用

• 批准号: 21380100
• 负责人: SHIRAISHI Susumu
• 金额: $ 11.23万
• 依托单位: Kyushu University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21380100/
• 关键词: クロマツ; マツ材線虫病; トランスクリプトーム; 抵抗性育種; 遺伝子発現; 植物; 遺伝子; 病理学; 抵抗性; ODD

The Japanese black pine(Pinus thunbergii) is highly susceptible to pine wilt disease caused by the pine wood nematode(PWN ; Bursaphelenchus xylophilus). To cope with this disease, researchers and tree breeders selected PWN-resistant individuals in a previous breeding program. In an attempt to understand the mechanisms of pathopoiesis and resistance, the mechanisms were clarified using LongSAGE(serial analysis of gene expression) and ODD(ordered differential display) analyses. Especially, by the global transcriptome analysis using SAGE, it is indicated that pathogenesis-related proteins, osmotin, lipoxygenase, chalcone synthase were significantly up-regulated, and eukaryotic translation initiation factor SUI1, translationally controlled tumor protein, and xyloglucan endotransglycosylase were down-regulated by the PWN inoculation in both the resistant and non-resistant pines. On the other hand, catalase, dienelactone hydrolase family protein were expressed at a higher level only in the resistant pine, pathogenesis-related proteins and leucoanthocyanidin dioxygenase were expressed only in the non-resistant pine. These differentially expressed genes are presumed to reflect some of the differences between the resistant and non-resistant pines.

日本黑松(Pinus Thunbergii)对松材线虫引起的松材线虫病高度敏感。为了应对这种疾病，研究人员和树木育种者在之前的育种计划中选择了抗PWN的个体。通过基因表达序列分析(LongSAGE)和有序差异显示(ODD)分析，阐明了致病和抗性的机制。特别是SAGE全转录组分析表明，无论是抗性松树还是非抗病松树，病程相关蛋白渗透素、脂氧合酶、查尔酮合成酶均显著上调，真核细胞翻译起始因子SUI1、翻译调控肿瘤蛋白和木葡聚糖内糖基转移酶均下调。另一方面，过氧化氢酶、双烯内酯水解酶家族蛋白仅在抗病松树中高水平表达，病程相关蛋白和亮蓝蛋白双加氧酶仅在非抗病松树中表达。这些差异表达的基因被认为反映了抗性和非抗性松树之间的一些差异。

项目成果

マツノザイセンチュウを接種されたクロマツ(Pinus thunbergii)の遺伝子発現プロファイル

接种黑松的黑松基因表达谱

• 发表时间: 2010
• 作者: Fukuzawa;K.;管蘭華・白石進
• 通讯作者: 管蘭華・白石進

SAGE法を用いた網羅的な発現遺伝子解析.マツ材線虫病抵抗性のメカニズム解明に向けて.

采用SAGE方法进行综合基因表达分析，阐明松材线虫抗病机制。

• 发表时间: 2012
• 期刊: 林木の育種
• 作者: 能勢美峰;白石進
• 通讯作者: 白石進

Comparison of the gene expression profiles of resistant and non-resistant Japanese black pine inoculated with pine wood nematode using a modified LongSAGE technique

• DOI: 10.1111/j.1439-0329.2010.00646.x
• 发表时间: 2011-04-01
• 期刊: FOREST PATHOLOGY
• 影响因子: 1.4
• 作者: Nose, M.;Shiraishi, S.
• 通讯作者: Shiraishi, S.