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Molecular systematical Studies of conifer using DNA sequence information

利用 DNA 序列信息对针叶树进行分子系统研究

基本信息

批准号：
09460070
负责人：
SHIRAISHI Susumu
金额：
$ 7.42万
依托单位：
Kyushu University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
1997
资助国家：
日本
起止时间：
1997 至 1998
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09460070/
关键词：
molecular systematics molecular Phylogeny conifer Coniferopsida chloroplast DNA DNA sequence nuclear DNA RAPD 球果網植物植物分類系統樹マツ科マツ属塩基配列情報球果綱植物 rbcL atpA petA

项目摘要

The molecular evolution of conifers was elucidated by using DNA sequence information on chloroplast DNA and/or 18S rDNA gene on nuclear DNA. The main results are as follows:1.Phylogeny of conifer at family level: The DNA sequences (ca, 3,000 bps) of three chloroplast DNA genes, atpA, petA and rbcL were decided in eight coniferous families. A new finding that was different from the traditional hypothesis was revealed from maximum likelihood analysis of sequence information.2.Phylogeny of the family Cupressanceae: The DNA sequence of the three above-mentioned genes was decided in 11 genera. Chamaecyparis nootkatensis which had been included to the genus Chamaecyparis up to now was classified into the genus Cupressus. In addition, the 18S rDNA sequences of six species in Chamaecyparis was decided. These species can identify using a specific sequence.3.Evolution of the family Pinaceae: The DNA sequences of six genes (atpA, petA, rbcL, rpoA, rps2 and rps3) on chloroplast DNA were decided for nine genera which composed the Pinaceae. The Phylogeny of the family was analyzed based on the sequence information. As a result, the family Pinaceae was classified into four subfamilies.4. The construction of a new classification system in the genus Pinus: In Pinus, the largest taxon in the conifer, the classification system based on the phylogeny was restructured from chloroplast DNA information. A molecular phylogenetic tree was made from DNA information of three genes (atpA, rbcL and rpoA) on chloroplast DNA. A new classification system which was greatly different from the traditional classification systems was proposed based on this tree. Four sections and 11 subsections of the new system were composed of the species which were distributed in the neighborhood. The biogeographic factor strongly influenced the evolution of the Pinus.

利用针叶树叶绿体DNA和细胞核DNA上的18S rDNA基因序列信息，阐明了针叶树的分子进化。主要研究结果如下：1。科水平上针叶树的系统发育：测定了8个针叶树科的3个叶绿体DNA基因atpA、petA和rbcL的DNA序列（约3000 bps）。通过对序列信息的极大似然分析，揭示了一个不同于传统假设的新发现。柏科系统发育：确定了11属上述3个基因的DNA序列。此前一直隶属于柏树属的诺特卡柏树被划入柏树属。此外，还确定了6个物种的18S rDNA序列。这些物种可以通过特定的序列来识别。松科植物的进化：确定了组成松科植物的9个属叶绿体DNA上6个基因（atpA、petA、rbcL、rpoA、rps2和rps3）的DNA序列。根据序列信息分析了该家族的系统发育。结果将松科划分为4个亚科。新分类系统的构建：在针叶树中最大的分类单元——松木中，利用叶绿体DNA信息重构了基于系统发育的分类系统。利用叶绿体DNA上3个基因（atpA、rbcL和rpoA）的DNA信息构建了分子系统发育树。在此基础上提出了一种与传统分类系统有较大区别的分类系统。新系统的4个区段和11个亚区段由分布在邻域的种组成。生物地理因素对松林的进化具有强烈的影响。