Establishment of the mouse immortalized erythroid precursor cell line using a temperature-sensitive SV40 large T antigen

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I examined a condition to produce human BclxL to mouse erythroid progenitor cells as a preliminary experiment about the production of the mouse immortalized erythroid progenitor cells. When the human BclxL expression plasmid was introduced into mouse erythroid progenitor cells by electroporation, it was revealed that expression of the human BclxL protein was very low. Then, when the promoter of the expression plasmid was changed into the EF-1alpha promoter instead of the CMV promoter, the expression level of human BclxL protein increased significantly.