Preparation of biomimetic prosthesis prepared from cultured smooth muscle cells and endothelial cells.

由培养的平滑肌细胞和内皮细胞制备仿生假体。

• 批准号: 01870100
• 负责人: TAKANO Tatsuya
• 金额: $ 2.69万
• 依托单位: Teikyo University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Developmental Scientific Research
• 财政年份: 1989
• 资助国家: 日本
• 起止时间: 1989 至 1991
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-01870100/
• 关键词: biomimetic prosthesis; collagen; endothelial cells; fibrinogen; fibronectin; formic acid; platelets; コラ-ゲン; フィブロネクチン; エラスチン

It is well know that arterial prostheses, especially those of small diameter, are often associated with thrombus formation and hyperplasia. In addition, it is necessary to retain the mechanical strength to withstand arterial pressure.A number of unsuccessful trials have been carried out using artificial prostheses made of Dacron or expanded polytetra fluoethylene (e-PTFE) seeded with endothelial cells to prevent thrombus formation and hyperplasia. To solve these problems, vein allograft and Dardik graft (umbilical vein treated with glutaraldehyde) have been tried. More recently, improved results have been seen when arterial allografts of the internal thoracic artery have been used in aortocoronary bypass operations. In view of this we considered that the arterial architecture may play a role in the prevention of hyperplasia and reistance to arterial pressure.In this study, a biomimetic prosthesis was prepared from canine arterial wall by extraction with formic acid. Under these conditions, the arterial architecture consisting of elastic lamella was conserved and the tensile strength was retained. Attachment of ^<51>Cr-endothelial cells to the surface of the prosthesis was enhanced in the presence of collagen, fibronectin, and fibrinogen. These agents also enhanced the adhesion of ^<51>Cr platelets. However, preattachment of endothelial cells to prostheses prevented platelet adhesion.This study demonstrates the production of a novel biomimetic arterial prosthesis that should prove useful, particularly in an investigation of the mechanism of thrombosis and hyperplasia.This work was supported in part by a grant from the Ministry of Education, Science and Culture of Japan.

众所周知，动脉假体，特别是那些小直径的，经常与血栓形成和增生有关。此外，还要保留承受动脉压的机械强度。许多不成功的试验使用涤纶或膨胀聚四氟乙烯（e-PTFE）制成的人工假体植入内皮细胞，以防止血栓形成和增生。为了解决这些问题，尝试了同种异体静脉移植和Dardik（戊二醛处理脐静脉）移植。最近，将同种异体胸椎内动脉移植到冠状动脉搭桥手术中，效果有所改善。鉴于此，我们认为动脉结构可能在预防增生和抵抗动脉压方面发挥作用。本研究采用甲酸萃取法制备犬动脉壁仿生假体。在这些条件下，由弹性片层组成的动脉结构得以保留，抗拉强度得以保留。在胶原蛋白、纤维连接蛋白和纤维蛋白原存在的情况下，^<51> cr -内皮细胞与假体表面的附着增强。这些药物还能增强^<51>Cr血小板的粘附。然而，内皮细胞预先附着在假体上可阻止血小板粘附。这项研究证明了一种新型仿生动脉假体的生产，特别是在血栓形成和增生机制的研究中，应该证明是有用的。这项工作得到了日本文部科学省的部分资助。

项目成果

K.Magari,H.Fukushima,S.Ishimaru,et al.: "Platelet adhesion to a biomimetic prothesis prepared from camine arterial wall." Artif.Organs. 15(6). 492-497 (1991)

K.Magari、H.Fukushima、S.Ishimaru 等人：“血小板粘附到由卡胺动脉壁制备的仿生假体上。”

Hirabayashi, J., and Kasai, K.: "Effect of amino acid substitution by site-directed mutagenesis on the carbohydrate recognition and stability of human14-kDa beta-galactoside-binding lectin." J. Biol. Chem.266. 23648-23653 (1991)

Hirabayashi, J. 和 Kasai, K.：“通过定点诱变进行氨基酸取代对人 14-kDa β-半乳糖苷结合凝集素的碳水化合物识别和稳定性的影响。”

Sakakura, Y., Hirabayashi, J., Oda, Y., Ohyama, Y., and Kasai, K.: "Structure of chicken 16-kDa beta-galactoside-binding lectin." J. Biol. Chem.265. 21573-21579 (1990)

Sakakura, Y.、Hirabayashi, J.、Oda, Y.、Ohyama, Y. 和 Kasai, K.：“鸡 16-kDa β-半乳糖苷结合凝集素的结构。”

Kasai, K., and Hirabayashi, J.: "Primary structures of vertebrate beta-galactoside-binding lectins." Lectins-Biology, Biochemistry, Clinical Biochemistry. 7. 69-75 (1990)

Kasai, K. 和 Hirabayashi, J.：“脊椎动物 β-半乳糖苷结合凝集素的主要结构。”

Y.Yagyu,R.Hashida,K.Iwasaki,et al.: "Effect of PGI_2 on platelet to partially denuded endothelial monolayer in vitro." Thromb.Res.64. 733-744 (1991)

Y.Yagyu、R.Hashida、K.Iwasaki 等人：“体外 PGI_2 对血小板至部分剥脱内皮单层的影响”。