Monoclonal Antibody Recognizing Peroxidized Lipoproteins Accumulated in Atherosclerotic Lesion.

在这项研究中，脂质过氧化参与了动脉粥样硬化病变，低密度脂蛋白的氧化修饰被认为参与了动脉粥样硬化的启动和促进。它们被巨噬细胞和平滑肌细胞吸收，导致胆固醇酯的大量积累。最近，我们开发了一种新的单克隆抗体（FOH1a/DLH3），它可以识别氧化LDL和MDA-LDL，乙酰化LDL。免疫组化分析显示，人冠状动脉粥样硬化病变中巨噬细胞衍生的泡沫细胞被抗体严重染色。抗体的表位可能是氧化磷脂酰胆碱（9CHO-PC），其中一些可能与多肽形成复合物。

In this research work, an involvement of lipid peroxide in atherosclerotic lesions was elucidated Oxidative modiffication of low density lipoproteins are believed to be involved in the intiation and promotion of atherosclerosis. They are taken up by macrophages and smooth muscle cells resulting in a massive accumulation of cholesteryl ester. Recently we developed novel monoclonal antibody (FOH1a/DLH3) which recognizes oxidized LDL but MDA-LDL,acetylated LDL.Immunohistochemical analysis in human coronary arteries showed that foam cells derived from macrophage in atherosclerotic lesions heavily stained by the antibody. Epitope of the antibody may be oxidized phosphatidylcholine (9CHO-PC), some of which may form complex with polypeptides.