Attempts for contruction of arterial wall with medial smooth muscle cells in vitro -An approach foam developmental biology-

体外用内侧平滑肌细胞构建动脉壁的尝试-泡沫发育生物学的方法-

• 批准号: 04557111
• 负责人: TAKANO Tatsuya
• 金额: $ 9.34万
• 依托单位: Teikyo University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Developmental Scientific Research (B)
• 财政年份: 1992
• 资助国家: 日本
• 起止时间: 1992 至 1994
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-04557111/
• 关键词: atherosclerosis; glycoprotein; vitronectin; extracellular matrix; cholesterol; embryo; arterial wall; 血管新生; 内皮細胞; エラスチン; 器官培養

Initial phase of smooth muscle differentiation in the mouse embryonic vascular system was observed immunohistochemically by using monoclonal antibody against alpha-smooth muscle actin. Little smooth muscle cells were detected in the vascular system of 9 day embryo, where only dorsal aorta and umbilical artery showed simptone of smooth muscle differentiation.In 10 day embryo, smooth muscle cells clearly recognized in the dorsal aorta, umbilical artery, omphalomesenteric artery, 3rd and 4th aortic arches and internal carotid artery.In 11 day embryo, smooth muscle cells formed 2-3 layrs at dorsal aorta, in 6th aortic arches, intersomitic arteries and other vessels.We tried to construct a novel in vitro culture system in order to elucidate induction process of smooth muscle differentiation. Aortic regions were dissected from 9 day mouse embryos and undifferentiated mesenchymal cells were cultured in medium with 10%FBS.After 3 days alpha-smooth muscle actin immunostaining developed in about 80% of cells. various growth factors, known as candidates for key substances were now examined in embryonic induction processes.

用抗α-平滑肌肌动蛋白单克隆抗体，对小鼠胚胎血管系统平滑肌分化的初期进行了免疫组化观察。9天胚胎的血管系统中几乎没有平滑肌细胞，仅在背主动脉和脐动脉处有平滑肌分化，10天胚胎的背主动脉、脐动脉、脐肠系膜动脉、第3、4主动脉弓和颈内动脉处有平滑肌细胞，11天胚胎的背主动脉处有2-3层平滑肌细胞，本研究试图建立一种新的体外培养体系，以阐明平滑肌细胞的诱导分化过程。从9天的小鼠胚胎中分离出主动脉区，将未分化的间充质细胞培养在含10%FBS的培养基中，3天后约80%的细胞出现α-平滑肌肌动蛋白免疫染色。现在在胚胎诱导过程中对各种生长因子进行了检查，这些生长因子被称为关键物质的候选物。

项目成果

今中 常雄: "lmmunological detection of Fl-ATPase subunit in rat liver peroxisomes." Biochem. Biophys. Res. Commun.195( 2). 1027-1034 (1993)

Tsuneo Imanaka：“大鼠肝脏过氧化物酶体中 Fl-ATP 酶亚基的免疫学检测。Biochem.195(2)”。

峰尾千恵子: "Angiotensin ll binding activity in cultured porcine arteridl endotherial cells." Biochem.Pharmacol.48(10). 1993-1995 (1994)

Chieko Mineo：“培养的猪动脉内皮细胞中的血管紧张素 II 结合活性。Biochem.Pharmacol.48(10) 1993-1995 (1994)。

T.Takano: "Accumulation of cholesteryl ester in atherosclerotic lesions." Acta Pathol.Jpn.42(9). 625-631 (1992)

T.Takano：“动脉粥样硬化病变中胆固醇酯的积累。”

今中常雄: "Immunological detection of Fl-ATPase subunit in rat liver peroxisomes." Biochem.Biophys.Res.Commun.195(2). 1027-1034 (1993)

Tsuneo Imanaka：“大鼠肝脏过氧化物酶体中 Fl-ATP 酶亚基的免疫学检测。” Biochem.Biophys.Res.Commun.195(2) (1993)。

峰尾千恵子: "Hydrolysis of lipid droplets in acid cholesteryl-esterase-deficient fibroblasts." Cell Struct.Funct.17. 191-196 (1992)

Chieko Mineo：“酸性胆固醇酯酶缺陷的成纤维细胞中脂滴的水解。”Cell Struct.Funct.191-196（1992）。