Attempts for contruction of arterial wall with medial smooth muscle cells in vitro -An approach foam developmental biology-

体外用内侧平滑肌细胞构建动脉壁的尝试-泡沫发育生物学的方法-

• 批准号: 04557111
• 负责人: TAKANO Tatsuya
• 金额: $ 9.34万
• 依托单位: Teikyo University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Developmental Scientific Research (B)
• 财政年份: 1992
• 资助国家: 日本
• 起止时间: 1992 至 1994
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-04557111/
• 关键词: atherosclerosis; glycoprotein; vitronectin; extracellular matrix; cholesterol; embryo; arterial wall; 血管新生; 内皮細胞; エラスチン; 器官培養

Initial phase of smooth muscle differentiation in the mouse embryonic vascular system was observed immunohistochemically by using monoclonal antibody against alpha-smooth muscle actin. Little smooth muscle cells were detected in the vascular system of 9 day embryo, where only dorsal aorta and umbilical artery showed simptone of smooth muscle differentiation.In 10 day embryo, smooth muscle cells clearly recognized in the dorsal aorta, umbilical artery, omphalomesenteric artery, 3rd and 4th aortic arches and internal carotid artery.In 11 day embryo, smooth muscle cells formed 2-3 layrs at dorsal aorta, in 6th aortic arches, intersomitic arteries and other vessels.We tried to construct a novel in vitro culture system in order to elucidate induction process of smooth muscle differentiation. Aortic regions were dissected from 9 day mouse embryos and undifferentiated mesenchymal cells were cultured in medium with 10%FBS.After 3 days alpha-smooth muscle actin immunostaining developed in about 80% of cells. various growth factors, known as candidates for key substances were now examined in embryonic induction processes.

通过使用针对α-光滑肌肉肌动蛋白的单克隆抗体，可以观察到小鼠胚胎血管系统平滑肌分化的初始阶段。在9天胚胎的血管系统中，很少检测到平滑肌细胞，那里只有背主动脉和脐动脉表现出平滑肌分化的simptone。在10天的胚胎中，平滑肌细胞在背主动脉，脐动脉，脐带，3RD和4th emberty，3rd和4th Arteric Arteric Artere Artere Muster中清楚地识别出平滑肌细胞。在第6个主动脉弓，肌间动脉和其他血管的背主动脉上形成2-3个Layrs。我们试图构建一种新型的体外培养系统，以阐明平滑肌分化的诱导过程。从9天的小鼠胚胎中解剖了主动脉区域，并在10％FBS的培养基中培养未分化的间充质细胞。在3天后，在约80％的细胞中出现了3天α-光滑肌肉肌动蛋白免疫染色。现在在胚胎诱导过程中检查了各种生长因子，称为关键物质的候选因子。

项目成果

高野達哉: "Active Oxygens,Lipid Peroxides,and Antioxidants" CRC Press,Boca Raton(ragi,K.,ed), 10 (1993)

Tatsuya Takano：“活性氧、脂质过氧化物和抗氧化剂”CRC Press，Boca Raton（ragi，K.，ed），10（1993）

T.Takano,H.Mowri,&H.Itabe: "Peroxidized lipoproteins in atherosclerotic lesions detectid by a monoclonal antibody" Active Oxygens,Lipid Peroxides,and Andtioxidants. 139-148 (1993)

T.高野，H.毛里，

峰尾 千恵子: "Angiotensin ll binding activity in cultured porcine arterial endotherial cells." Biochem.Pharmacol.48( 10). 1993-1995 (1994)

Chieko Mineo：“培养的猪动脉内皮细胞中的血管紧张素 II 结合活性。”Biochem.Pharmacol.48(10) 1993-1995 (1994)。

今中 常雄: "lmmunological detection of Fl-ATPase subunit in rat liver peroxisomes." Biochem. Biophys. Res. Commun.195( 2). 1027-1034 (1993)

Tsuneo Imanaka：“大鼠肝脏过氧化物酶体中 Fl-ATP 酶亚基的免疫学检测。Biochem.195(2)”。

峰尾千恵子: "Angiotensin ll binding activity in cultured porcine arteridl endotherial cells." Biochem.Pharmacol.48(10). 1993-1995 (1994)

Chieko Mineo：“培养的猪动脉内皮细胞中的血管紧张素 II 结合活性。Biochem.Pharmacol.48(10) 1993-1995 (1994)。