Oxidized lipoproteins released from atherosclerotic lesions

动脉粥样硬化病变释放的氧化脂蛋白

基本信息

批准号：
12672121
负责人：
TAKANO Tatsuya
金额：
$ 2.43万
依托单位：
Teikyo University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2000
资助国家：
日本
起止时间：
2000 至 2001
项目状态：
已结题

项目摘要

We developed a sandwich ELISA method to measure OXLDL levels using a novel anti-OXLDL monoclonal antibody (FOHla/DLH3) and an anti-apolipoprotein B polyclonal antibody. FOHla/DLH3 is specific for ox-LDL and does not bind to native, acetylated, MDA-treated, or glycated LDL.With the use of this new and highly sensitive method, we measured the levels of OXLDL in patients with acute myocardial infarction (AMI), unstable angina pectoris (UAP), and stable angina pectoris (SAP). In addition, we immuno-histochemically studied the presence of oxLDL in coronary atherectomy specimens taken from the culprit lesions responsible for SAP and UAP, although oxLDL levels in these patients were not available. Hence, the study is based on a cohort of patients with similar clinical characteristics but in whom different methodologies were applied. The first group contains patients presenting with various degrees of signs and symptoms of a coronary syndrome in whom oxLDL levels were determined and correlated with the severity of the syndrome. The second group of patients also presented with angina pectoris, but in those patients, only atherectomy specimens obtained from culprit lesions were available and studied immuno-histochemically for the presence of oxLDL.In conclusions, this study demonstrates that OXLDL levels show a significant positive correlation with the severity of acute coronary syndromes and that the more severe lesions also contain a significantly higher percentage of OXLDL positive macrophages. These observations suggest that increased levels of OXLDL relate to plaque instability in human coronary atherosclerotic lesions.

我们开发了一种三明治ELISA方法，使用新型的抗OXLDL单克隆抗体（FOHLA/DLH3）和抗磷脂B多克隆抗体测量OXLDL水平。 Fohla/dlh3是特异性的，不与本机，乙酰化，MDA处理或糖化的LDL结合。使用这种新的且高度敏感的方法，我们测量了急性心肌（AMI），Unclable Angina angina angina pectoris（uap）（Uap）（Uap）（uap）和Stable Angua angin Angua angin Angin Angin Angin Angin Angin Angin Angin Angin Angin Angin Angin AnginA（STOBLE）（STOBLE），STABLE（STABLE），STABLE（UAP）和Stable AnginA（STOBLE），STABLE（STABLE），STABLE（UAP），STABLE（STABLE）。此外，我们通过免疫 - 历史化学研究了OXLDL在负责SAP和UAP的罪魁祸首的冠状动脉动脉科切除术标本中的存在，尽管这些患者的OXLDL水平尚无。因此，该研究基于具有相似临床特征但使用不同方法的患者队列。第一组包含冠状动脉综合征的各种体征和症状的患者，其中确定了OXLDL水平并与综合征的严重程度相关。第二组患者也出现了心绞痛，但是在那些患者中，只有从罪魁祸首获得的动脉切除术标本可用，并在结论中进行了免疫 - 归化性，并研究了免疫 - 归纳化学，这项研究表明，OXLDL水平与急性脉络膜综合综合症的较高量相关性显着较高，这一点显着，这些相关性显着，而巨大的coron氏菌群则具有显着的正相关。巨噬细胞。这些观察结果表明，OXLDL水平的升高与人冠状动脉粥样硬化病变中的斑块不稳定性有关。