Development of a novel method detecting interaction between proteins and DNAs

开发检测蛋白质和 DNA 之间相互作用的新方法

• 批准号: 23657119
• 负责人: ITO Yasuhiro
• 金额: $ 1.75万
• 依托单位: National Agriculture and Food Research Organization
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Challenging Exploratory Research
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-23657119/
• 关键词: 遺伝学; 核酸; 蛋白質; 発現制御; 分子認識; 転写因子; DNA; 相互作用; 分子間相互作用

To develop a sensitive method detecting interaction of proteins with their binding DNAs, we applied a real-time PCR based methods with probe DNA-conjugated antibodies to a protein and also the target DNA conjugated another probe DNA. We detected interaction between a protein and the target DNAs by the method, but the sensitivity was much lower than that by the other authentic method. Thus, additional improvement is required for the practical use of this new method.

为了开发一种灵敏的检测蛋白质与其结合DNA相互作用的方法，我们应用了基于实时PCR的方法，其中探针DNA缀合抗体与蛋白质以及靶DNA缀合另一探针DNA。我们用该方法检测了蛋白质与靶DNA之间的相互作用，但灵敏度远低于其他可靠的方法。因此，这种新方法的实际应用需要额外的改进。