A direct calorimetric system for the continuous recording of heat balance of freely moving small animals.

一个系统已经设计了一个提供的系统，用于在受限制的喂养计划下自由移动的种子的同样的测量。该系统包括专门设计的热量计、空气供应器和流量调节器、空气湿度控制器、热沉降温度调节热移动器、湿度传感器或平台、O_2和CO_2分析器以及数据记录器和个人计算机。超过所有的反应时间和热量计的敏感性小于5分钟和0.499 W/mV，热量计的强度为25 ° C。热产量估计是根据O_2消费量和CO_2生产量的连续测量进行的，以及用个人计算机计算的热平衡中的变化。使用此系统，以下结果已获得。（1）在不同的肽组中注入的肽的效果，在不同的方向中，热量损失的反应。（2）温度调节反应对身体变暖的存在有明显的日常差异，当老鼠身体受到限制时，但当他们被允许自由移动时。（3）持续和持续的热量暴露的影响是不同的，以反映热量暴露的模式对大鼠产生热量暴露的影响。（4）受限制的老鼠（一天只喂养2小时）增加轮子运行活动的几个小时，喂食前和喂食后。车轮运行和平均热量生产之间的关系在前和后喂食期间和控制预先储存期间之间并没有区别。5） Fasting suppresses heat loss response to intravenous injections of endotoxin and endogenous pyrogens but not to intracerebroventricular injections。其他一些实验也是有条件的。

A system has been designed which provides for simultaneous measurements of heat loss and heat production of freely moving rodents under restricted feeding schedules. The system includes specially designed calorimeter, an air suppier and flow regulators, air remperature controllers, a thermobath for heat sink temperature regulation, humidity sensing devices or a platemeter, O_2 and CO_2 analyzers and a data logger and a personal computer. The over-all response time and the sensitivity of the calorimeter are less than 5 min and 0.499 W/mV at a calorimeter remperature of 25゜c. Heat production was estimated from continuous measurements of O_2 consumption and CO_2 production, and changes in heat balnce were computed with the personal computer. Using this system, following results were obtained. 1) Effects of peptides injected into the cerebroventricle are in different directions in heat loss response with different peptide-groups. 2) A clear daily difference in temperature regulatory responses to body warming exists when rats are physically restrained, but not when they were allowed to move freely. 3) The effects of intermittent and continuous heat exposures are different according to the mode of heat exposure to obtain heat acclimation in rats. 4) Food restricted rats (2 hours feeding only in a day) enhance wheel running activity several hours before and after the feeding time. The relationship between wheel running and average heat production was not different between pre- and post feeding periods and control ad-lib feeding period. 5) Fasting suppresses heat loss response to intravenous injections of endotoxin and endogenous pyrogens but not to intracerebroventricular injections. Several other experiments were also conducted.