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A direct calorimetric system for the continuous recording of heat balance of freely moving small animals.

直接量热系统，用于连续记录自由活动的小动物的热平衡。

基本信息

批准号：
61870009
负责人：
NAGASAKA Tetsuo
金额：
$ 4.61万
依托单位：
Kanazawa University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Developmental Scientific Research
财政年份：
1986
资助国家：
日本
起止时间：
1986 至 1988
项目状态：
已结题

项目摘要

A system has been designed which provides for simultaneous measurements of heat loss and heat production of freely moving rodents under restricted feeding schedules. The system includes specially designed calorimeter, an air suppier and flow regulators, air remperature controllers, a thermobath for heat sink temperature regulation, humidity sensing devices or a platemeter, O_2 and CO_2 analyzers and a data logger and a personal computer. The over-all response time and the sensitivity of the calorimeter are less than 5 min and 0.499 W/mV at a calorimeter remperature of 25゜c. Heat production was estimated from continuous measurements of O_2 consumption and CO_2 production, and changes in heat balnce were computed with the personal computer. Using this system, following results were obtained. 1) Effects of peptides injected into the cerebroventricle are in different directions in heat loss response with different peptide-groups. 2) A clear daily difference in temperature regulatory responses to body warming exists when rats are physically restrained, but not when they were allowed to move freely. 3) The effects of intermittent and continuous heat exposures are different according to the mode of heat exposure to obtain heat acclimation in rats. 4) Food restricted rats (2 hours feeding only in a day) enhance wheel running activity several hours before and after the feeding time. The relationship between wheel running and average heat production was not different between pre- and post feeding periods and control ad-lib feeding period. 5) Fasting suppresses heat loss response to intravenous injections of endotoxin and endogenous pyrogens but not to intracerebroventricular injections. Several other experiments were also conducted.

设计了一个系统，它提供了同时测量的热损失和热生产的自由移动的啮齿动物在有限的喂养时间表。该系统包括专门设计的热量计、空气供应器和流量调节器、空气温度控制器、用于调节热沉温度的恒温槽、湿度传感装置或板计、O_2和CO_2分析仪、数据记录仪和个人计算机。在25 ℃的温度下，总响应时间小于5 min，灵敏度小于0.499W/mV。通过连续测量O_2消耗量和CO_2产生量来估算产热量，并利用个人计算机计算热平衡的变化。使用该系统，获得了以下结果。1)脑室注射不同肽类对热损失反应的影响方向不同。2)当大鼠被身体约束时，对体温升高的温度调节反应存在明显的每日差异，但当它们被允许自由活动时则不存在。3)间歇热暴露和连续热暴露对大鼠热适应的影响因热暴露方式不同而不同。4)食物限制的大鼠（每天仅喂食2小时）在喂食时间前后几小时增强转轮跑步活动。投饵前后和对照自由投饵期的转轮运行与平均产热的关系无显著差异。5)禁食抑制对内毒素和内源性热原静脉注射的热损失反应，但不抑制对脑室内注射的热损失反应。还进行了其他一些实验。