权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

Thermocline for the study of behavioral thermoregulation in small animals

用于研究小动物行为温度调节的温跃层

基本信息

批准号：
05557005
负责人：
NAGASAKA Tetsuo
金额：
$ 5.31万
依托单位：
Kanazawa University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Developmental Scientific Research (B)
财政年份：
1993
资助国家：
日本
起止时间：
1993 至 1995
项目状态：
已结题

项目摘要

The purpose of this study was to design a thermocline for accurate measurements of preferred ambient temperature of small animals such as rats in various conditions. The dimensions of the thermocline were 200 x 16 x 18 (H) cm, whose both ends were welded with cupper tubings in which cold and hot water was independently circulated and a thermal, gradient was maintained along the axis of the thermocline. With this instrument the preferred temperature of rats was estimated. Body temperature was continuously measured by telemetry. 1) Behavioral thermoregulation and its diurnal change during fever. The preferred temperature was higher in the inactive phase and lower in the active phase. After a pyrogen was injected the preferred temperature rose before the riso of body temperature and fell before the thermolysis began. 2) Behavioral thermoregulation in FOK rats. Body and preferred temperatures were always lower in FOK rats. The critical temperatures for the increase in metabolic rate and for the skin vasodilatation were also lower. 3) Endotoxin shock. A large dose of endotoxin caused a fall in body temperature as well as the preferred temperature. 4) Behavioral thermoregulation after feeding. Shortly after feeding the rats selected a lower ambient temperature. 5) In the rats naive to the experimental condition maintained higher body and preferred temperatures for at least 3 days. 6) Effects of restraint and exercise. During restraint, the rats maintained higher body temperature and the critical temperatures for thermoregulatory responses. In exercise-trained rats nonevaporative heat loss was always higher than control rats.

本研究的目的是设计一种温跃层，用于精确测量小动物（如大鼠）在各种条件下的首选环境温度。温跃层的尺寸为200 × 16 × 18（H）cm，两端焊接有铜管，冷水和热水在铜管中独立循环，并沿温跃层的轴线保持沿着热梯度。用该仪器估计了大鼠的偏好温度。通过遥测连续测量体温。1)行为体温调节及其在发热时的昼夜变化。优选的温度在非活性相中较高，在活性相中较低。注射致热原后，首选温度在体温上升前上升，在治疗开始前下降。2)FOK大鼠的行为体温调节。FOK大鼠的体温和偏好温度总是较低。代谢率增加和皮肤血管舒张的临界温度也较低。3)内毒素休克。大剂量的内毒素导致体温下降以及首选温度。4)进食后的行为体温调节。喂食后不久，大鼠选择了较低的环境温度。5)在未接受实验条件的大鼠中，保持较高的体温和首选温度至少3天。6)克制和锻炼的效果。在束缚期间，大鼠保持较高的体温和体温调节反应的临界温度。在运动训练的大鼠非蒸发热损失总是高于对照组大鼠。