Investigating the mechanism of placental amnion-chorion membranes on periodontal regeneration

胎盘羊膜绒毛膜促进牙周再生的机制研究

• 批准号: 21K16974
• 负责人: 吉田 航
• 金额: $ 3万
• 依托单位: Tokyo Dental College
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Early-Career Scientists
• 财政年份: 2021
• 资助国家: 日本
• 起止时间: 2021-04-01 至 2024-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-21K16974/
• 关键词: 歯周組織再生療法; ヒト胎盤由来羊膜・絨毛膜; 成長因子

2022年度の研究実績In vivoでは，10週齢のWistarラットの上顎第一臼歯近心に規格化欠損を形成後ルートプレーニングし，歯周組織欠損を作製した。HACM＋HPC応用群，HPC単独群，Unfilled群に分け，術後4週で形態学的 (マイクロCT)，組織学的(H-E染色)にて検討した。2021年度では術後2週での評価を行い，マイクロCT画像による歯槽骨形成量の解析では，HACM+HPC群で新生骨様構造を認めた。さらに，骨梁構造解析でHACM+HPC群はHPC，Unfilled群と比較し骨体積率で有意に高い値を示した。2022年度では術後4週での評価を行い，HACM+HPC群はHPC，Unfilled群と比較し骨体積率で有意に高い値を示した。術後4週のH-E染色においてHACM+HPC 群では，Unfilled，HPC群と比較し，新生骨様構造が多く観察され，術後2週と同様の傾向を示した。また,免疫組織化学染色(VEGF，α-SMA)を行い血管新生についても評価を行った。In vitroでは，HACMをPBS中に溶解したHACM extract (100-500 ug/ml)を作製し，ELISAにてVEGFおよびFGF-2の濃度を測定した。ラットより採取した歯根膜由来細胞の培養液中にHACM extractを滴下し，細胞増殖能をWST-1，細胞遊走能をWound healing assayにより評価した。2022年度も引き続き同様の評価を行い，VEGFおよびFGF-2の成長因子が含めれている100 ug/ml のHACM Extractは，Control群と比較して歯根膜由来細胞の増殖および遊走の促進を認めた。以上のことからHACMの含有成分が歯根膜由来細胞の増殖および遊走を促すことで，歯周組織治癒を促進させることが示唆された。

The results of the research in 2022 are In vivo. After 10 weeks of normalization of the maxillary first mortar near the center of the palate, the tissue loss of the periphery of the jaw was produced. HACM+HPC used group, HPC single group, Unfilled group are divided into three groups. Morphological (CT) and histological (H-E staining) were analyzed 4 weeks after surgery. In 2021, the evaluation of 2 weeks after surgery, the analysis of the amount of trough bone formation in the CT image of the patient, and the recognition of the new bone structure of the HACM+HPC group were carried out.さらに, bone beam structure analysis, HACM+HPC group, HPC, Unfilled group, comparison, bone volume ratio, high intention, high value, and high performance. In 2022, four weeks after surgery, the evaluation of the operation, the HACM+HPC group, the HPC, and the Unfilled group were compared, and the bone volume rate was high and the bone volume rate was high. H-E staining of HACM+HPC group 4 weeks after operation, Unfilled, HPC group comparison, and new bone structure inspection, 2 weeks after operation, the same tendencies are shown.また, immunohistochemical staining (VEGF, α-SMA) を行いangiogenesis についても evaluation価を行った. In vitro, HACM was dissolved in PBS, HACM extract (100-500 ug/ml) was produced, and the concentration of VEGF and FGF-2 was measured by ELISA. The HACM extract was dropped into the culture medium of cells derived from root membranes collected by Raton, and the cell proliferation ability was WST-1, and the cell migration ability was evaluated by Wound healing assay. 2022 annual growth factor VEGF growth factor 100 ug/ml HACM Extract, Control group, Comparison, Propagation of cells from which the root membrane originates, and recognition of migration and promotion. The above-mentioned HACM contains ingredients that promote the proliferation and migration of cells derived from the root membrane, and promotes the healing of peripheral tissues.

项目成果

To evaluate the effect of Human dehydrated amnion-chorion membranes on periodontal healing in vivo and in vitro.

评价人脱水羊膜绒毛膜在体内和体外对牙周愈合的影响。

• 发表时间: 2021
• 作者: Imamura K;Hamada Y;Yoshida W;Yoshikawa K;Saito A
• 通讯作者: Saito A

Investigating the Effects of Dehydrated Human Amnion-Chorion Membrane on Periodontal Healing.

• DOI: 10.3390/biom12060857
• 发表时间: 2022-06-20
• 期刊: Biomolecules
• 影响因子: 5.5

ヒト胎盤由来羊膜・絨毛膜メンブレン（HACM）の応用が歯周組織の創傷治癒に及ぼす影響

人胎盘源性羊膜绒毛膜（HACM）应用对牙周组织创面愈合的影响

• 发表时间: 2021
• 作者: Imamura K;Hamada Y;Yoshida W;Murakami T;Nakane-Koyachi S;Yoshikawa K;Saito A.;今村健太郎，中根咲，吉田航，吉川幸輝，齋藤淳
• 通讯作者: 今村健太郎，中根咲，吉田航，吉川幸輝，齋藤淳