TNF刺激による血管内皮細胞の過酸化水素産生に及ぼす麻酔薬の影響

麻醉剂对 TNF 刺激的血管内皮细胞过氧化氢产生的影响

• 批准号: 08771178
• 负责人: 岩城 久美
• 金额: $ 0.64万
• 依托单位: Toyama Medical and Pharmaceutical University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Encouragement of Young Scientists (A)
• 财政年份: 1996
• 资助国家: 日本
• 起止时间: 1996 至 无数据
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-08771178/
• 关键词: 活性酸素; 麻酔薬; 血管内皮

ウシ頸動脈より得た継代培養血管内皮細胞(国立衛生試験所細胞バンク)を,20%ウシ胎児血清加イ-グルMEM培地で,5%CO2,15%air,37℃の環境下で培養した.培養血管内皮細胞の上清を,臨床使用濃度とその100倍の高濃度のpropofol,thiopentone,midazolam,ketamineを含むHBSSlmlで完全に置換し,1時間pre-incubationした.麻酔薬負荷後の培養血管内皮細胞の上清を,5μMDCFH-DA(膜透過性過酸化水素感受性蛍光色素)を含むHBSSlmlで完全に置換し、5分間pre-incubationした.共焦点レーザー顕微鏡を用い,培養血管内皮細胞を488nmのレーザー光で励起,530nmで蛍光測定をしながら,THFα20ng/mlを含むHBSSlmlを負荷し,TNF投与前後の血管内皮細胞内過酸化水素濃度の変化を測定した.Ketamine投与下では,血管内皮細胞内酸化ストレスは抑制される傾向を認めた.本研究は,ketamineの脳保護作用のメカニズム解明に,貢献する可能性がある.

The culture of vascular endothelial cells (cells from the National Institute of Health), 20% fetal serum supplemented with MEM, 5% CO2, 15% air and 37 ℃ incubation were obtained. The supernatant of vascular endothelial cells was cultured, and the temperature of the bed was 100 times higher than that of high temperature propofol,thiopentone,midazolam,ketamine, which contained HBSSlml completely, and pre-incubation was used in 1 time. After loading, the supernatant of vascular endothelial cells was cultured. 5 μ m of MDCFH-DA (membrane permeation through acidified water receptor photopigment) contained HBSSlml completely and pre-incubation in 5 minutes. Confocal microarray was used to culture vascular endothelial cells (488nm), 530nm photometry (530nm), THF α 20ng/ml (HBSSlml), TNF before and after vascular endothelial cell acidification. Ketamine was used to determine the intracellular acidified water content, and vascular endothelium intracellular acidification inhibited the activation of vascular endothelial cells. In this study, ketamine plays an important role in protecting and explaining the possibility.