Reprogramming of translation in response to abiotic stress from model plant to crop

响应从模型植物到作物的非生物胁迫的翻译重编程

• 批准号: 418142664
• 负责人: Dr. Sotirios Fragkostefanakis, Ph.D.
• 金额: --
• 依托单位: Fakultät Angewandte Naturwissenschaften
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2019
• 资助国家: 德国
• 起止时间: 2018-12-31 至 2022-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/418142664?language=en
• 关键词: Reprogramming; translation; response; abiotic; stress

We have clear indication that the initial reactions and even the intermediate response of plants to abiotic stresses follows a general mechanism. Our preliminary studies uncovered first components likely involved in these early responses to heat and microgravity stress. According to our observations, SnRK1 and TOR signaling are candidates for a regulatory point of convergence between different abiotic stress responses. Further elucidation of the processes downstream of SnRK1 and TOR will provide important insights into principles of early stress response reactions generally occurring in plants. Our long term goal is to describe the common molecular mechanisms occurring early during abiotic stress response in plants with respect to different plant species and different stresses. Further, we aim to define the point of divergence of the different pathways in response to different abiotic stresses. In the first funding period, we will focus on the two stresses for which tools are well established in the laboratory. This includes the natural occurring heat stress and the human enforced stress microgravity. The information obtained will be important for the definition of the individual stress response paths and at the same time allow the formulation of general stress response mechanisms upstream of the stress specific reactions. Our specific aims are:A) Elucidation of translational reprogramming as initial reaction and intermediate res-ponse to heat and microgravity stress by probing the ribosome assembled mRNAs in very short time windows after stress application in S. lycopersicum and A. thaliana to define the timing and characteristics of proteome reprogramming B) Elucidation of differential splicing as initial reaction and intermediate response to heat stress by mRNA sequencing and proteome analysis in S. lycopersicum and A. thaliana to define the functional reprogramming of the proteomeC) Analysis of phosphorylation changes as initial reaction and intermediate response to heat stress and microgravity in S. lycopersicum and A. thaliana to define and compare the signaling pathways involved.

我们有明确的迹象表明，植物对非生物胁迫的初始反应，甚至中间反应遵循一个一般的机制。我们的初步研究发现了可能参与这些对热和微重力应力的早期反应的第一种成分。根据我们的观察，SnRK1和TOR信号是不同非生物胁迫反应之间的调节点的候选者。对SnRK1和TOR下游过程的进一步阐明将为植物中通常发生的早期胁迫反应的原理提供重要的见解。我们的长期目标是针对不同的植物物种和不同的胁迫，描述在植物非生物胁迫响应早期发生的共同分子机制。此外，我们的目标是定义不同的途径在不同的非生物胁迫的分歧点。在第一个资助期，我们将重点关注实验室中已建立工具的两种压力。这包括自然发生的热应力和人类强制应力微重力。所获得的信息将是重要的定义的个人压力响应路径，并在同一时间允许制定一般的压力响应机制的压力特异性反应的上游。我们的具体目标是：A）通过在应力施加后的非常短的时间窗口内探测核糖体组装的mRNA，阐明翻译重编程作为热和微重力应力的初始反应和中间反应。lycopersicum和A. B）通过mRNA测序和蛋白质组分析阐明了差异剪接作为热胁迫的初始反应和中间反应。lycopersicum和A. C）分析S. thaliana对热胁迫和微重力的初始反应和中间反应的磷酸化变化。lycopersicum和A. thaliana来定义和比较所涉及的信号通路。