ヒト肥満細胞の分化に関する研究

人类肥大细胞分化的研究

• 批准号: 09771389
• 负责人: 黒崎 貞行
• 金额: $ 0.58万
• 依托单位: Nippon Medical School
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Encouragement of Young Scientists (A)
• 财政年份: 1997
• 资助国家: 日本
• 起止时间: 1997 至 1998
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-09771389/
• 关键词: ヒト; 肥満細胞; 分化; マスト細胞; 鼻アレルギー; 前駆細胞; stem cell factor; サイトカイン

鼻アレルギー患者において鼻粘膜内の肥満細胞増多が特徴的である。これまでに、肥満細胞は造血幹細胞に由来し、未熟な前駆細胞として骨髄から末梢血中に流出することが確認され、その分化成熟因子としてStem Cell Factor(SCF)が明らかにされた。また、我々は、鼻アレルギー患者の鼻粘膜上皮から産生されるSCFは正常者に比べ有意に多いことを報告した。これらの事実から、肥満細胞の前駆細胞は鼻粘膜局所で分化成熟すると推測し、本実験を計画した。まず、正常者3名と鼻アレルギー患者2名を対象とし、末梢血から単核細胞をとりだしSCF(50ng/ml)とともに10^6の細胞濃度で培養した。正常群、アレルギー群ともに、2週間後に20〜30のコロニーが発育した。いずれもコロニー中に占める好塩基性細胞は5〜13%であり、トリプターゼ陽性細胞が4〜7%認められ、両者間で差がみられなかった。すなわち、末梢血中には肥満細胞の前駆細胞が存在し、その数には正常者とアレルギー患者との間に大きな差がみられなかった。次に、4例の手術で採取した鼻粘膜を細切し、組織中に存在する単核細胞だけをとりだし、SCFを含まないもの、SCF濃度50ng/ml、100ng/mlの3つの条件下で培養した。いずれも培養4週後には、濃度にかかわらずSCF存在下では全く細胞の増殖が見られず、SCFを含まないものでは、繊維芽細胞が著名に増殖した。以上の結果からは、肥満細胞の分化は鼻粘膜局所でおこり、その環境因子の違い(SCFの濃度)により局所肥満細胞増多が生ずるという我々の考えと一致しなかった。その理由として、末梢血に比べて前駆細胞数が少ない、局所での分化成熟因子にはSCF以外のものも関与する、また、肥満細胞は鼻粘膜以外の場所で成熟し、何れかのサイトカインの働きによって局所に集まる可能性などが考えられた。また、本実験をとおして、新たな知見としてSCFが繊維芽細胞の抑制因子となる可能性も示唆された。

There are many hypertrophic cells in the nasal mucosa of the patients with nasal dysentery. The origin of hematopoietic cells, immature progenitor cells, bone marrow cells, peripheral blood flow, differentiation and maturation factor Stem Cell Factor (SCF). The patients with nasal mucosa epithelium, normal SCF were more likely to report than those with normal nasal mucosa. In the first place, the differentiation and maturation of the nasal mucosa bureau was discussed, and the plan was designed. In 3 normal and normal patients, there were 2 patients with rhinorrhea, and 2 patients with peripheral blood, peripheral blood, nuclear cells, SCF (50ng/ml), 10 ^ 6 cells and 6 cells. The normal group, the normal group and the normal group. It accounts for 5%, 13%, 4%, 7%, 4%, 7%, 4%, 7%, 7%, 7%, 7%, 7%, 7%, 5%, 13%, 7%, 7%, 5%, 13%, 7%, 7%, 7%, 5%, 13%, 4%, 7%, 4%, 7%, 4%, 7%, 4%, 7%, 4%, 7%, 4%, 7%, 4%, 7%, 7%, 7%, 7%, 7%, 7%, 7%, 7%, 7%, 7%, 7%, 7%, 7%, 7%, 7%, 7%, 7%, 7%, 7%, 7%, 7%, 7%, 5%, 13%, 7%, 7%, 7%, 7%, 7% The presence of fat cells in peripheral blood, the number of normal patients and patients. In the second stage, the nasal mucosa was excised in 4 cases, and the tissue was cultured under the condition of the presence of cell cycle, SCF, SCF, 100ng/ml and so on. After 4-year-old culture, the whole plant cell colonization was observed in the presence of SCF, and the SCF was well-known in the presence of cell culture. As a result of the above results, the differentiation of fat cells in the nasal mucosa Bureau and the environmental factors in the nasal Mucosal Bureau (SCF) were consistent with the results of the above results. The number of cells in peripheral blood is lower than that in previous ones, the number of cells in peripheral blood is less than that in previous ones, the number of cells in peripheral blood is lower than that in previous ones, the number of cells in peripheral blood is lower than that in previous ones, the number of cells in peripheral blood is lower than that in previous ones, the number of cells in peripheral blood is lower than that in previous ones, the number of cells in peripheral blood is lower than that in previous ones, the number of cells in peripheral blood is lower than that in previous ones, the number of cells in peripheral blood is lower than that in previous ones, the number of cells in peripheral blood is lower than that in previous ones, the number of cells in peripheral blood is smaller than that in previous ones, the number of cells in peripheral blood is smaller than that in previous ones. The information on the inhibition factor of cell growth, the possibility of SCF maintenance, and the new information indicate that it is possible to abet the situation.