400 MHz NMR-Konsole

400 MHz 核磁共振控制台

• 批准号: 420143102
• 金额: --
• 依托单位: Philipps-Universität Marburg
• 依托单位国家: 德国
• 项目类别: Major Research Instrumentation
• 财政年份: 2019
• 资助国家: 德国
• 起止时间: 2018-12-31 至 无数据
• 项目状态: 未结题
• 来源: https://gepris.dfg.de/gepris/projekt/420143102?language=en
• 关键词: 400; MHz; NMR; Konsole

In order to meet the increased requirements on the nmr spectroscopy due to the projects described in ´Beiblätter zur Forschung´ (amongst others within the LOEWE center DRUID), a spectrometer console, a sensitive multicore probe head and a high-capacity sample changer shall be purchased. The new acquisition will not only increase the sample capacity but in particular enable the measurement of samples with very low concentration, the expansion of measurements of hetero nuclei as well as the determination of protein dynamics by means of 19F nmr spectroscopy. The aims of the research projects comprise the development of active substances against neglected tropical diseases (e.g.schistosomiasis), parasitic diseases, highly pathogenic viruses and bacteria, as well as design and synthesis of selective inhibitors of methyl transferase, serine proteases, of plasmin and matriptase. Further aims are the characterization of thermodynamic and binding kinetic parameters of enzymes as well as the determination of the rearrangement of configuration during ligand binding. Furthermore, the development of biosensors, the identification of sulfur-containing secondary metabolites of the genus Allium, as well as the discovery of the biosynthetic potential of fungi and the investigation of their secondary metabolisms are pursued.

为了满足“Beiblätter zur Forschung”（LOEWE中心DRUID内的其他项目）中所述项目对核磁共振波谱的增加要求，应购买光谱仪控制台，敏感的多核探头和高容量样品转换器。新的采集装置不仅增加了样品容量，而且特别能够测量极低浓度的样品，扩大了异核的测量范围，以及通过19F核磁共振波谱法测定蛋白质动力学。这些研究项目的目的包括研制防治被忽视的热带病（如血吸虫病）、寄生虫病、高致病性病毒和细菌的活性物质，以及设计和合成甲基转移酶、丝氨酸蛋白酶、纤溶蛋白和基质酶的选择性抑制剂。进一步的目的是表征酶的热力学和结合动力学参数，以及确定配体结合过程中构型的重排。此外，生物传感器的发展，含硫葱属植物次生代谢产物的鉴定，以及真菌生物合成潜力的发现和次生代谢的研究也将继续进行。