Upgrade 600 MHz solid-state NMR console including MAS probe head

升级 600 MHz 固态 NMR 控制台，包括 MAS 探头

• 批准号: 420478124
• 金额: --
• 依托单位: Heinrich-Heine-Universität Düsseldorf
• 依托单位国家: 德国
• 项目类别: Major Research Instrumentation
• 财政年份: 2019
• 资助国家: 德国
• 起止时间: 2018-12-31 至 无数据
• 项目状态: 未结题
• 来源: https://gepris.dfg.de/gepris/projekt/420478124?language=en
• 关键词: Upgrade; 600; MHz; solid; state

We apply for an exchange console for a solid-state NMR spectrometer, including an ultrafast MAS probe head facilitating MAS frequencies up to 111 kHz to address following research topics:Structural and functional investigation of amyloid fibrils of the disease relevant proteins IAPP, Aß(1-42) and PrP: We aim to elucidate tertiary and quarternary structure of these amyloid fibrils by high-resolution solid-state NMR-spectroscopy, study ligand binding of small molecules to the surface of the fibrils. Structural rearrangements of fibrils upon the change of pH and salt concentration will be determined. Further, we will characterize the interaction between oligomeric Aß(1-42) and natively folded PrP on the molecular level. Finally, the biofilm formation of the functional amyloid TasA which can be studied in monomeric, oligomeric and as mature amyloid fibrils will be characterized in detail by determining the structures of the oligomeric and the fibrillar form.Besides classical solid-state NMR experiments involving 13C and 15N detection, we will also apply experiments based on 1H detection. For this purpose, a high-speed MAS probe head facilitating spinning speeds up to 111 kHz is mandatory. Besides experiments for resonance assignment and structure determination we will also apply and improve experiments for investigation of local dynamics. For this purpose, the membrane protein OmpX will serve as a model substance.

我们申请了一个固态核磁共振光谱仪的交换控制台，包括一个超快的MAS探头，促进MAS频率高达111 kHz，以解决以下研究主题：疾病相关蛋白IAPP，A？(1-42)和PrP的淀粉样原纤维的结构和功能研究：我们的目标是通过高分辨率固体核磁共振谱阐明这些淀粉样原纤维的三级和四级结构，研究小分子与纤维表面的配体结合。将确定纤维在pH和盐浓度变化时的结构重排。此外，我们将在分子水平上表征寡聚体A？(1-42)与天然折叠PrP之间的相互作用。最后，通过测定低聚体和纤维形态的结构，对功能性淀粉样蛋白TASA的生物膜形成进行详细的表征。除了涉及13C和15N检测的经典固态核磁共振实验外，我们还将应用基于1H检测的实验。为此，必须配备高速MAS探头，以促进高达111 kHz的旋转速度。除了共振归属和结构确定的实验外，我们还将应用和改进局部动力学研究的实验。为此，膜蛋白OmpX将作为模型物质。