Upgrade 600 MHz solid-state NMR console including MAS probe head

我们申请了一个固态NMR光谱仪的交换控制台，包括一个超快MAS探头，可将MAS频率提高到111 kHz，以解决以下研究课题：疾病相关蛋白IAPP，April（1-42）和PrP的淀粉样蛋白原纤维的结构和功能研究：我们的目标是通过高分辨率固态NMR光谱来阐明这些淀粉样纤维的三级和四级结构，研究小分子与原纤维表面的配体结合。将测定pH和盐浓度变化时原纤维的结构重排。此外，我们将在分子水平上表征寡聚腺苷酸（1-42）和天然折叠的PrP之间的相互作用。最后，生物膜形成的功能性淀粉样蛋白TasA，可以在单体，低聚物和成熟的淀粉样蛋白原纤维的研究将其特征在于详细确定的低聚物和原纤维形式的结构。除了经典的固态NMR实验，涉及13 C和15 N检测，我们还将应用实验的基础上1H检测。为此，必须使用高速MAS探头，使旋转速度达到111 kHz。除了共振分配和结构确定实验，我们还将应用和改进实验，以调查当地的动态。为此，膜蛋白OmpX将作为模型物质。

We apply for an exchange console for a solid-state NMR spectrometer, including an ultrafast MAS probe head facilitating MAS frequencies up to 111 kHz to address following research topics:Structural and functional investigation of amyloid fibrils of the disease relevant proteins IAPP, Aß(1-42) and PrP: We aim to elucidate tertiary and quarternary structure of these amyloid fibrils by high-resolution solid-state NMR-spectroscopy, study ligand binding of small molecules to the surface of the fibrils. Structural rearrangements of fibrils upon the change of pH and salt concentration will be determined. Further, we will characterize the interaction between oligomeric Aß(1-42) and natively folded PrP on the molecular level. Finally, the biofilm formation of the functional amyloid TasA which can be studied in monomeric, oligomeric and as mature amyloid fibrils will be characterized in detail by determining the structures of the oligomeric and the fibrillar form.Besides classical solid-state NMR experiments involving 13C and 15N detection, we will also apply experiments based on 1H detection. For this purpose, a high-speed MAS probe head facilitating spinning speeds up to 111 kHz is mandatory. Besides experiments for resonance assignment and structure determination we will also apply and improve experiments for investigation of local dynamics. For this purpose, the membrane protein OmpX will serve as a model substance.