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600 MHz NMR Instrument Upgrade at Northwestern University

西北大学 600 MHz NMR 仪器升级

基本信息

批准号：
8247293
负责人：
Ishwar Radhakrishnan
金额：
$ 26.74万
依托单位：
NORTHWESTERN UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
2012
资助国家：
美国
起止时间：
2012-04-01 至 2013-03-31
项目状态：
已结题

项目摘要

DESCRIPTION (provided by applicant): Funding is requested for replacing the console of a high-resolution 600 MHz NMR spectrometer at Northwestern University's Biological NMR Center, a unique resource providing structural and chemical biologists access to high-field instrumentation within a single facility for inter- disciplinary research. Since 1990, with support from NIH and NSF, Northwestern researchers have established and maintained an outstanding resource for macromolecular NMR that has had a significant positive impact on the research programs of multiple investigators both within the college and the university at large. The 600 MHz spectrometer, which is the one and only NMR instrument in the facility, is heavily and continuously used. However, the reliability of this instrument has decreased lately because of a range of problems (including a most persistent FIFO underflow error problem) associated with an aging console, which was last replaced in 1999. The acquisition of a new console along with a replacement compressor (aka cold pack) for the cold probe would cater to the heavy demand for NMR time by a user base that comprises seven well-funded major research groups working on a variety of basic and clinical problems in biology or at the chemistry-biology interface. The research topics being studied include structure-function analyses of transcription factors, structural and functional genomics of infectious agents, electron transfer via protein-protein interactions, structural characterization of natural products, structural biology of kinesin regulation, design and development of novel inhibitors and contrast agents for magnetic resonance imaging and diagnostic applications, and novel synthetic chemistry approaches for medicinal applications. The projects have strong structural and/or functional foci and all of them will benefit from the added sensitivity (mainly from improvements in hardware that have occurred in the past decade), but most significantly, added reliability afforded by a new console and cold probe compressor. The proposed instrument will have all the capabilities to perform any contemporary biomolecular NMR pulse sequence experiment. Since the magnet and cold probe (other than the compressor) have been determined to be in good shape, there are no plans to replace either of them. The new console will have four channels with the first channel for 1H excitation, decoupling and detection, a second channel for excitation, decoupling and broadband detection of biologically-relevant heteronuclei, and a dual channel for excitation and decoupling of biologically-relevant heteronuclei, and pulsed field gradient accessories.

描述（由申请人提供）：请求资助更换西北大学生物核磁共振中心的高分辨率 600 MHz 核磁共振波谱仪控制台，该中心是一个独特的资源，为结构生物学家和化学生物学家提供在单一设施内使用高场仪器进行跨学科研究的机会。自1990年以来，在支持下在 NIH 和 NSF 的支持下，西北大学的研究人员建立并维护了出色的大分子 NMR 资源，这对学院和整个大学内多个研究人员的研究项目产生了重大的积极影响。 600 MHz 光谱仪是该设施中唯一的核磁共振仪器，被大量且持续地使用。然而，由于与 1999 年最后一次更换的老化控制台相关的一系列问题（包括最持久的 FIFO 下溢错误问题），该仪器的可靠性最近有所下降。购买新控制台以及用于冷探头的替换压缩机（又名冷包）将满足用户群对 NMR 时间的巨大需求，该用户群由七个资金雄厚的主要研究小组组成，致力于解决各种基础和临床问题在生物学或化学-生物学界面。正在研究的研究主题包括转录因子的结构功能分析、感染因子的结构和功能基因组学、通过蛋白质-蛋白质相互作用的电子转移、天然产物的结构表征、驱动蛋白调节的结构生物学、用于磁共振成像和诊断应用的新型抑制剂和造影剂的设计和开发，以及用于医学应用的新型合成化学方法。这些项目具有强大的结构和/或功能重点，所有这些项目都将受益于增加的灵敏度（主要来自过去十年中硬件的改进），但最重要的是，新的控制台和冷探针压缩机提供的增加的可靠性。拟议的仪器将具备执行任何当代生物分子核磁共振脉冲序列实验的所有功能。由于磁铁和冷探头（压缩机除外）已确定状态良好，因此不打算更换其中任何一个。新控制台将有四个通道，其中第一个通道用于1H激发、解耦和检测，第二个通道用于生物相关异核的激发、解耦和宽带检测，双通道用于生物相关异核的激发和解耦，以及脉冲场梯度附件。