Plastid translation and plant development

质体翻译和植物发育

• 批准号: 42308154
• 负责人: Professor Dr. Ralph Bock
• 金额: --
• 依托单位: Max-Planck-Institut für molekulare Pflanzenphysiologie (MPI-MP)
• 依托单位国家: 德国
• 项目类别: Research Units
• 财政年份: 2007
• 资助国家: 德国
• 起止时间: 2006-12-31 至 2013-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/42308154?language=en
• 关键词: Plastid; translation; plant; development

By using a combination of transplastomic and pharmacological approaches, work in the current funding period has established novel tools for studying the retrograde signals generated from plastid translation. Genetic and pharmacological studies have indicated that plastid genome-encoded protein(s) serve as generator of the retrograde signal that determines leaf shape in response to plastid translational activity. Mutant analyses have revealed that the retrograde signal is xanthoxin, a precursor of the phytohormone abscisic acid (ABA), and have suggested a molecular mechanism for how the translational activity of the chloroplast is converted into the metabolic signal that triggers the phenotypic responses. Future research aims to rigorously test our current working model using biochemical and genetic approaches. These will comprise comparative analyses of available ABA mutants, plastid translation mutants and transplastomic lines as well as overexpression and RNAi-mediated downregulation of candidate components in the signal transduction chain. Furthermore, the (de)regulation of nuclear genes in response to the signal will be analyzed with transcript and metabolite profiling techniques to (i) reveal new nuclear target genes that are controlled by this retrograde pathway and (ii) identify additional signalling components.

通过使用transplastomic和药理学的方法相结合，在目前的资助期内的工作已经建立了新的工具，用于研究从质体翻译产生的逆行信号。遗传学和药理学研究表明，质体基因组编码的蛋白质作为逆行信号的发生器，决定叶片形状响应质体翻译活性。突变体分析表明，逆行信号是黄嘌呤，植物激素脱落酸（阿坝）的前体，并提出了一种分子机制，叶绿体的翻译活动是如何转化为代谢信号，触发表型反应。未来的研究旨在使用生物化学和遗传学方法严格测试我们目前的工作模型。这些将包括比较分析可用的阿坝突变体，质体翻译突变体和transplastomic线，以及过表达和RNAi介导的下调候选成分的信号转导链。此外，将使用转录本和代谢物分析技术分析响应于信号的核基因的（去）调节，以（i）揭示由这种逆行途径控制的新的核靶基因，以及（ii）鉴定其他信号传导组分。