A fluorescent hinge peptide for the dimerization of proteins through disulfide formation

一种荧光铰链肽，用于通过二硫键形成使蛋白质二聚化

• 批准号: 427497450
• 负责人: Professor Dr. Armin Geyer
• 金额: --
• 依托单位: Fachgebiet Organische Chemie
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2019
• 资助国家: 德国
• 起止时间: 2018-12-31 至 2022-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/427497450?language=en
• 关键词: fluorescent; hinge; peptide; dimerization; proteins

We recently described a disulfide-bridged dimeric peptide which forms through oxidative folding of the 12 amino acids motif CX3CX2CX3C (C = cysteine, X = other amino acids). The high cysteine content – every third amino acid is cysteine – exceeds that of natural cysteine motifs and results in a unique homodimerization selectivity upon air oxidation. Not only the isolated peptide undergoes covalent dimerization but also proteins up to a size of 150 kDa that are fused to the peptide by recombinant DNA technology. One of the homodimeric peptide shows blue fluorescence that is probably caused by the excimer formation between its two tryptophans. We will investigate the structural requirements of this effect that increases the application potential of this hinge dimers as fluorescence tags. The molecular requirements of the dimerization process and the oxidative folding will be studied by systematic structural modifications. We will try to identify intermediates of the balance act of oxidative folding of eight cysteines by complementary synthetic and analytic strategies. The structural requirements of the dimerization process will be tested by systematic amino acid variations at the cysteines, the turn amino acids, and the side chain charges with a special focus on the heterodimeric peptides. Spectroscopic methods will be used to characterize the conformational mobility of the two halves of the hinge from its response to systematic variations of the structural environment i.e. the “loading” of the hinge with polypeptides of different size. The central aim of this application is to identify the structural prerequisites of the tetradisulfide dimeric peptide for its high tolerance to different protein loads and its unique regioselectivity of oxidative folding. The investigation of physicochemical properties, conformation, and cooperative mobility of this novel protein dimerization motif will be of interest for peptide/protein folding and stability in general.

我们最近描述了一种通过氧化折叠12个氨基酸基序CX3CX2CX3C形成的二硫桥接二聚体肽（C =半胱氨酸，X =其他氨基酸）。高半胱氨酸含量——每三种氨基酸中就有半胱氨酸——超过了天然半胱氨酸基元，在空气氧化时具有独特的二聚选择性。不仅分离的肽经历共价二聚化，而且通过重组DNA技术将150 kDa大小的蛋白质融合到肽中。其中一种同型二聚体肽显示蓝色荧光，这可能是由它的两个色氨酸之间的准分子形成引起的。我们将研究这种效应的结构要求，增加这种铰链二聚体作为荧光标记的应用潜力。二聚化过程和氧化折叠的分子要求将通过系统的结构修饰来研究。我们将尝试通过互补的合成和分析策略来确定8种半胱氨酸氧化折叠平衡行为的中间体。二聚化过程的结构要求将通过系统的半胱氨酸、转氨基酸和侧链电荷的氨基酸变化来测试，并特别关注异二聚肽。光谱学方法将用于表征铰链两半的构象迁移率，从其对结构环境的系统变化的响应，即铰链与不同大小的多肽的“负载”。本应用程序的中心目的是确定四二硫二聚肽的结构先决条件，因为它具有对不同蛋白质负载的高耐受性和独特的氧化折叠区域选择性。这种新型蛋白质二聚化基序的理化性质、构象和协同迁移性的研究将对肽/蛋白质折叠和稳定性产生重要影响。