Analytical ultracentrifuge

自生物物理化学研究所和相关的结构生物化学研究机构成立以来，汉诺威一直是具有国际地位的分析超速离心法(AUC)的所在地。我们的AUC设备不仅是MHH感兴趣的各方的重要研究工具，而且也被全国许多科学家使用。借助AUC，可以在不受固相干扰的情况下，对蛋白质、核酸和配体在溶液中的相互作用进行定性和定量研究。目前正在使用的设备是2008年的Beckman Coulter ProteomeLab XL-I，不再是最先进的。在运行的第12年，它的使用越来越受到停机、高维修和不断增加的维护成本的限制。新的Beckman Coulter Optima AUC型号将光学元件从真空室中移出。这促进了高性能光学组件的集成，并实现了多达三种不同光学元件的同时检测。以更高的扫描速度和分辨率，可以在多达20个波长检测到沉淀。这使得对具有不同吸收光谱的分子的定量相互作用研究得到改进。较高的时间分辨率有利于对敏感蛋白质的研究，并能够显著提高选择性地追踪较大蛋白质复合体的沉淀。

Since the foundation of the Institute for Biophysical Chemistry and the associated research facility for Structural Biochemistry, Hannover has been a location for analytical ultracentrifugation (AUC) of international standing. Our AUC equipment is an important research tool not only for interested parties at MHH, but it is also used by many scientists nationwide. With the help of AUC, interactions of proteins, nucleic acids and ligands can be qualitatively and quantitatively investigated in solution without the influence of interfering solid phases. The device currently in use, a Beckman Coulter ProteomeLab XL-I from 2008, is no longer state-of-the-art. In the 12th year of operation, its usage is increasingly restricted by downtimes, high repair and increasing maintenance costs. The new Beckman Coulter Optima AUC model has the optical components moved out of the vacuum chamber. This facilitates the integration of high-performance optical components and enables simultaneous detection with up to three different optics. Sedimentation can be detected at up to 20 wavelengths with higher scan speed and resolution. This allows improved quantitative interaction studies of molecules with different absorption spectra. The higher temporal resolution facilitates the investigation of sensitive proteins and enables the tracking of the sedimentation of larger protein complexes with significantly increased selectivity.