Analytical ultracentrifuge

分析超速离心机

• 批准号: 429433916
• 金额: --
• 依托单位: Medizinische Hochschule Hannover (MHH)
• 依托单位国家: 德国
• 项目类别: Major Research Instrumentation
• 财政年份: 2020
• 资助国家: 德国
• 起止时间: 2019-12-31 至 无数据
• 项目状态: 未结题
• 来源: https://gepris.dfg.de/gepris/projekt/429433916?language=en
• 关键词: Analytical; ultracentrifuge

Since the foundation of the Institute for Biophysical Chemistry and the associated research facility for Structural Biochemistry, Hannover has been a location for analytical ultracentrifugation (AUC) of international standing. Our AUC equipment is an important research tool not only for interested parties at MHH, but it is also used by many scientists nationwide. With the help of AUC, interactions of proteins, nucleic acids and ligands can be qualitatively and quantitatively investigated in solution without the influence of interfering solid phases. The device currently in use, a Beckman Coulter ProteomeLab XL-I from 2008, is no longer state-of-the-art. In the 12th year of operation, its usage is increasingly restricted by downtimes, high repair and increasing maintenance costs. The new Beckman Coulter Optima AUC model has the optical components moved out of the vacuum chamber. This facilitates the integration of high-performance optical components and enables simultaneous detection with up to three different optics. Sedimentation can be detected at up to 20 wavelengths with higher scan speed and resolution. This allows improved quantitative interaction studies of molecules with different absorption spectra. The higher temporal resolution facilitates the investigation of sensitive proteins and enables the tracking of the sedimentation of larger protein complexes with significantly increased selectivity.

自生物物理化学研究所和相关的结构生物化学研究机构成立以来，汉诺威一直是国际分析超浓缩（AUC）的所在地。 我们的AUC设备是一个重要的研究工具，不仅对MHH感兴趣的各方，但它也被全国许多科学家使用。在AUC的帮助下，可以定性和定量研究溶液中蛋白质，核酸和配体的相互作用，而不受干扰固相的影响。目前使用的设备是2008年生产的Beckman Coulter ProteomeLab XL-I，已不再是最先进的设备。在运行的第12年，其使用越来越受到停机时间、高维修率和不断增加的维护成本的限制。新的Beckman Coulter Optima AUC型号将光学组件移出真空室。这有利于高性能光学元件的集成，并能够同时检测多达三个不同的光学器件。可在多达20个波长下检测沉积物，扫描速度和分辨率更高。这允许改进具有不同吸收光谱的分子的定量相互作用研究。更高的时间分辨率有利于敏感蛋白质的研究，并能够以显着增加的选择性跟踪较大蛋白质复合物的沉降。