Studies on the pathogenesis of tubulo-nephrosis or -nephritis due to infectious

感染性肾小管肾病或肾炎发病机制的研究

• 批准号: 01480101
• 负责人: ITAKURA Chitoshi
• 金额: $ 4.35万
• 依托单位: Hokkaido University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (B)
• 财政年份: 1989
• 资助国家: 日本
• 起止时间: 1989 至 1991
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-01480101/
• 关键词: Infectious bronchitis virus; Nephritis; Coronavirus; Budding; Tracheitis; Pneumonia; Chicken; 鶏; 伝染性気管支炎; 伝染性気管支炎ウイルス; 間質性腎炎; 成鶏; 痛風; 尿細管腎症; 尿細管・間質性腎炎

Tubulo-nephrosis or -nephritis due to infectious bronchitis virus (IBV) has occurred mostly in broiler chickens less than 3 weeks of age in Japan. The morbidity reached occasionally into about 10%.In 1991, we encountered tubulo-nephrosis or -nephritis in 151- and 158-day-old layer chickens on a farm located in Hokkaido. We isolated an IBV (HB-976) from them. When HB-976 strain was inoculated into day-old SPF chicks, more than 90% of them died between 4 and 8 days postinoculation. The dead birds showed histologically acute tubulonephrosis, tracheitis and pneumonia. Electronmicroscopically, the urinary tubular epithelial cells revealed swollen mitochondria with vacuolation, increase in polysomes, and increase in membrane-limitted vacuoles. IBV particles were frequent in their cytoplasm or vacuoles, showing budding from the membrane of the cytoplasmic vacuoles. Some of these involved cells were necrotic, while others remained revealing the viral replication. Immunohistochemically, IBV antigens were demonstrated in the urinary tubular epithelia.From the results obtained here, it was concluded that the tubulonephrosis was initiated with viral replication. However, the viral replication in the tubular epithelia continued for a long time without the cell destruction. In addition, it was recognized that a virulent IBV strain is present in the field chickens.

由传染性支气管炎病毒(IBV)引起的小管肾病或肾炎在日本主要发生在3周龄以下的肉鸡身上。发病率有时高达10%左右。1991年，我们在北海道的一个养殖场遇到了151和158日龄蛋鸡的肾小管肾病或肾炎。我们从他们身上分离出一株IBV(HB-976)。用HB-976株接种1日龄SPF雏鸡，在接种后4~8天内，90%以上的雏鸡死亡。死鸟的组织学表现为急性肾小管肾病、气管炎和肺炎。电子显微镜下，尿小管上皮细胞线粒体肿胀，空泡化，多聚体增多，膜限制的空泡增多。IBV颗粒多见于细胞质或空泡内，从细胞质空泡的膜上萌发。其中一些参与的细胞是坏死的，而另一些仍然揭示了病毒的复制。免疫组织化学显示，IBV抗原存在于尿路小管上皮细胞中，由此得出结论：肾小管肾病是由病毒复制引起的。然而，病毒在肾小管上皮细胞中的复制持续了很长一段时间，没有细胞破坏。此外，人们还认识到在田间鸡群中存在一种IBV强毒株。

项目成果

Chen,B.Y.et al.: "A virulent infections bronchitis virus isolated from layer chickens" J.Vet.Med.Sci.54. (1992)

Chen,B.Y.等人：“从蛋鸡中分离出的强毒感染性支气管炎病毒”J.Vet.Med.Sci.54。

Chen, B. Y. et al.: "Ultrastructural studies of urinary tubules infected with infectious bronchitis virus" Avian Pathol.21 (1992)

Chen, B. Y. 等人：“传染性支气管炎病毒感染的尿管的超微结构研究”Avian Pathol.21 (1992)

Chem,B.Y.et al.: "Ultra structural studies of urinary tubules infected with infections bronchitis virus" Avian pathol.21. (1992)

Chem，B.Y.等人：“感染支气管炎病毒的尿管的超结构研究”禽病理学21。

Chen, B. Y. et al.: "A virulent infectious bronchitis virus isolated from layer chickens" J. Vet. Med. Sci.54 (1992)

Chen, B. Y. 等人：“从蛋鸡中分离出的一种强毒传染性支气管炎病毒”J. Vet。