Characters, replication patterns and pathogenicity of a calicivirus isolated from rabbits

兔杯状病毒的特征、复制模式及致病性

• 批准号: 04454112
• 负责人: ITAKURA Chitoshi
• 金额: $ 4.16万
• 依托单位: Hokkaido University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (B)
• 财政年份: 1992
• 资助国家: 日本
• 起止时间: 1992 至 1993
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-04454112/
• 关键词: Calicivirus; Rabbit hemorrhagic disease; Virus antigen; Immunostain; Virus particles; Hepatitis; 壊死性肝炎; 播種性血管内凝固; ウイルス複製; 白血球毒性; 肝臓

A rabbit hemorrhageic disease (RHD) virus was isolated from Korean rabbits. The purified virus particles were 35 to 40 nm in diameter and had hollow depressions on their surface. The structural protein of the virus was composed of a single major polypeptide of 63 kDa.This virus had the high virulence and caused acute deaths in rabbits. Histopathological lesions caused by RHD virus were acute necrotizing hepatitis consisting of focal or solitary necrosis of hepatocytes.Immunohistochemically, RHD virus antigens were detected in degenrated and necrotic hepatocytes in formalin fixed tissues from rabbits infected with RHD virus. The findings indicated that RHD virus has a predilection for hepatocytes and replicates in the cytoplasm of the hepatocytes. The virus antigens were also detected in the endothelial cells and macrophages of the spleen.Ultrastructurally, a large number of virus particles resembling a calicivirus in size and morphology were demonstrated in the cytoplasm of many necrotic hepatocytes. The particles were accumulated mainly in the vaculoes of the necrotic hepatocytes.We isolated a virus from the liver of RHD in China. The characteristics and pathogenicity of the virus were the same as those of the Korean virus.From the results obtained here, the calicivirus isolated from RHD was hugh virulent and had an affinity faor adult rabbit hepatocytes.

从韩国兔中分离出兔出血性疾病（RHD）病毒。纯化后的病毒颗粒直径为35～40 nm，表面有中空凹陷。该病毒的结构蛋白由63 kDa的单一主要多肽组成。该病毒毒力较高，可引起家兔急性死亡。 RHD病毒引起的组织病理学病变为急性坏死性肝炎，表现为肝细胞局灶性或孤立性坏死。免疫组化检测感染RHD病毒的家兔福尔马林固定组织中退化、坏死的肝细胞中检测到RHD病毒抗原。研究结果表明，RHD病毒对肝细胞有偏好，并在肝细胞的细胞质中复制。脾脏内皮细胞和巨噬细胞中也检测到了病毒抗原。超微结构显示，许多坏死肝细胞的细胞质中存在大量大小和形态类似杯状病毒的病毒颗粒。这些颗粒主要积聚在坏死肝细胞的空泡中。我们从中国RHD的肝脏中分离到病毒。该病毒的特征和致病性与韩国病毒相同。从本研究结果来看，从RHD中分离到的杯状病毒具有很强的毒力，并且对成年兔肝细胞有亲和力。

项目成果

Park,J.H.: "Detection of rabbit haemorrhagic disease virus particles in the rabbit liver tissues" Journal of Comparative Pathology. 107. 329-340 (1992)

Park，J.H.：“兔肝组织中兔出血性疾病病毒颗粒的检测”比较病理学杂志。

Park, J.H.: "Detection of rabbit haemorrhagic disease virus particles in the rabbit liver tissues" J.Comp.Pathol. 107. 329-340 (1992)

Park, J.H.：“兔肝组织中兔出血性疾病病毒颗粒的检测”J.Comp.Pathol。

Park,J.-H.et al.: "Detection of rabbit haemorrhagic disease virus particles in the rabbit liver tissues" Journal of Comparative Pathology. 107. 329-340 (1992)

Park,J.-H.等人：“兔肝组织中兔出血性疾病病毒颗粒的检测”比较病理学杂志。

Park,J.H.: "Detection of rabbit haemorrhagic diesease virus antigen in tissues by immunohistochemistry" Research in Veterinary Science. 52. 299-306 (1992)

Park，J.H.：“通过免疫组织化学检测组织中的兔出血性疾病病毒抗原”兽医学研究。

Ueda,K.et al.: "Disseminated intravascular coagulation (DIC) in rabbit haemorrhagic disease" Japanese Journal of Veterinary Research. 40. 133-141 (1992)

Ueda,K.et al.：“兔子出血性疾病中的弥散性血管内凝血（DIC）”日本兽医研究杂志。