Biological and pathological significance of neurofilaments in nerve cell - using neurofilament deficient quails

神经细胞中神经丝的生物学和病理学意义——利用神经丝缺陷鹌鹑

• 批准号: 07456127
• 负责人: ITAKURA Chitoshi
• 金额: $ 4.93万
• 依托单位: Hokkaido University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 1995
• 资助国家: 日本
• 起止时间: 1995 至 1996
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07456127/
• 关键词: Neurofilament; Quail; Cytoskelton; Neurotoxin; Axonal change; 2,5hexanedione; Neuropathology; Animal model; 軸索症; 2,5-hexanadiome; モデル動物; 神経変性; アクリルアミド; TOCP; 疾患モデルル動物

We discovered first a primary axonal disease affecting the central and peripheral nervous system in a new mutant strain of the Japanese quail, which was named Quv. This strain has no neurofilament (NF) in the axons or neuronal cell bodies in the whole body. Quv is kept in our laboratory.Regeneration of myelinated fibers in the sciatic nerve 2 weeks after crush injury was studied morphometrically in Quv and normal quail (controls). There were fewer regenerated myelinated fibers per nerve distal to the crush site in Quv than in controls. There were fewer myelin lamellae in relation to axonal area in Quv than in controls. The results indicate a retardation of both radial growth of axons and myelination around axons of the same size in Quv compared with controls. Such retardation may be explained by the deficiency of NF.We have been examined the affection of the neurotoxin with an accumulation of NF,IDPN and acrylamide. Another neurotoxin, 2,5-hexanedione (HD) was injected into Quv and control quails. As a result, axonal swellings with NF accumulation in the sciatic nerve occurred in controls, but not in Quv. Therefore, all neurotoxins inducing the NF accumulation are not reactive in Quv.

我们首次在一种新的突变品系日本鹌鹑中发现了一种影响中枢和外周神经系统的原发轴突疾病，命名为Quv。该菌株在全身的轴突或神经元胞体中均未见神经丝(NF)。Quv保存在本实验室。对挤压伤后2周的有髓神经再生进行了形态计量学研究。与对照组相比，QUV组挤压部位远端每条神经再生的有髓神经纤维较少。QUV组髓鞘板层与轴突面积之比明显少于对照组。结果表明，与对照组相比，Quv组轴突的径向生长和相同大小的轴突周围的髓鞘形成都受到了抑制。这种迟缓可以用NF缺乏来解释。我们已经研究了神经毒素对NF、IDPN和丙烯酰胺积累的影响。将另一种神经毒素2，5-己二酮(HD)注入Quv和对照组。结果，对照组出现轴突肿胀并伴有坐骨神经中的神经纤维积聚，而在Quv组则没有。因此，所有诱导核因子积聚的神经毒素在Quv中都不是反应性的。

项目成果

Jiang, X. X.: "Regeneration of myelinated fiber after crush injury is retarded in sciatic nerves of mutant Japanese quails deficient in neurofilaments" Acta Neuropathol.92. 467-472 (1996)

Jiang, X. X.：“缺乏神经丝的突变日本鹌鹑的坐骨神经在挤压损伤后有髓纤维的再生受到阻碍”Acta Neuropathol.92。

Takahashi,A.: "Acrylamide-induced peripheral neuropathy in normal and neurofilament-deficient Japanese quail." Acta Neuropathol.89(1). 17-22 (1995)

Takahashi,A.：“正常和神经丝缺陷的日本鹌鹑中丙烯酰胺引起的周围神经病变。”

Jiang, X. M.: "Regeneration of myelinated fiber after crush injury is retarded in sciatic nerves of mutant Japanese quails deficient in neurofilaments" Acta Neuropathol.92. 467-472 (1996)

Jiang, X. M.：“缺乏神经丝的突变日本鹌鹑的坐骨神经在挤压损伤后有髓纤维的再生受到阻碍”Acta Neuropathol.92。

Hasegawa, H., Murayama, T., Takahashi, A., Itakura, C.and Nomura, Y: "Changes of GTB binding proteins not neurofilament-associated proteins, in the brain of the neurofilament deficient quail." Neurochem.Int. 28 (2). 221-229 (1996)

Hasekawa, H.、Murayama, T.、Takahashi, A.、Itakura, C. 和 Nomura, Y：“神经丝缺陷鹌鹑大脑中 GTB 结合蛋白的变化，而不是神经丝相关蛋白的变化。”

Zhao, J. X.: "Smaller axon and unaffected numbers of microtubules to number of myelin lamellae of myelinated fibers in the mutant quail deficient in neurofilaments." Acta Neuropathol.89. 305-313 (1995)

赵，J. X.：“在神经丝缺陷的突变鹌鹑中，轴突较小，微管数量与有髓纤维髓鞘层数量不受影响。”