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Studies on Diagnosis, Treatment and Protection of Cryptosporidiosis in Animal and Man

动物和人隐孢子虫病的诊断、治疗和防护研究

基本信息

批准号：
63860039
负责人：
ITAKURA Chitoshi
金额：
$ 5.38万
依托单位：
Hokkaido University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Developmental Scientific Research (B).
财政年份：
1988
资助国家：
日本
起止时间：
1988 至 1990
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-63860039/
关键词：
Cryptosporidium Oocyst Protozoon OPG Chicken Calf Immunity 器官培養免疫蛍光法スポロゾイトキニヨン染色

项目摘要

Cryptosporidial infections have occurred in man and various kinds of animals and birds in many countries. Cryptosporidial oocysts are very small in size. Therefore, it is difficult to detect the oocysts in the feces. We used Sheather's sugar floatation method for detecting the oocysts in feces from chickens and calves. A specific gravity of the sugar fluid recommended was 1.20 for calves and 1.25 for chickens. In addition, a method for OPG value was determined.Oocysts isolated from chickens and calves were observed morphologically. As a result, the chicken oocysts were different from Cryptosporidium (C) meleagridis and C. baileyi. On the other hand, the calf oocysts resembled C. Parvum. The morphology of oocysts was available for identification of the species.There is currently no known effective therapy and protection for cryptosporidiosis. However, an immunogenicity for this parasite was suggested. Namely, birds experimentally infected with the parasite did not develop the infection from 8 weeks after the first infection. Therefore, immunological treatment and diagnosis should be studied on this condition.We achieved an organ culture of the bursa of Fabricii from 4-week-old chickens. This culture is useful for observation of the life cycle and screening of medicines, because cryptosporidium in chickens infects prominently the epithelium of this organ.

隐孢子虫感染已在许多国家的人和各种动物和鸟类中发生。隐孢子虫卵囊体积非常小。因此，很难检测粪便中的卵囊。用Sheather氏糖漂浮法检测鸡、犊牛粪便中的卵囊。建议的糖液比重为小牛1.20，鸡1.25。此外，还建立了测定OPG值的方法，并对分离的鸡和犊牛卵囊进行了形态学观察。结果表明，鸡卵囊与隐孢子虫和隐孢子虫不同。贝利伊。另一方面，小牛卵囊类似于C。Parvum。隐孢子虫病目前尚无有效的治疗和预防措施。然而，这种寄生虫的免疫原性提出。也就是说，用寄生虫实验感染的鸟在第一次感染后8周内没有发生感染。因此，应研究这种情况下的免疫治疗和诊断。我们对4周龄鸡的腔上囊进行了器官培养。由于鸡隐孢子虫主要感染该器官的上皮细胞，因此该培养物可用于观察生命周期和筛选药物。