Study on virulence factors of Vibrio parahaemolyticus

本研究旨在探讨副溶血性弧菌引起胃肠炎的致病机制。通过本实验获得了以下结果：1）从人分离的神奈川酚非酮阴性副溶血性弧菌中发现了一种Vp-TDH相关溶血素（命名为VP-TRH）作为毒力因子。2）证实了霍乱弧菌非51型、拟态弧菌和霍氏弧菌可产生Vp-TDH/TRH相关毒素。对这些VP-TDH/TRH家族毒素进行了纯化和鉴定。3）用单克隆抗体和多克隆抗体对VP-TDH/TRH的免疫学特性进行了分析。表达生物学。5）通过制备嵌合毒素，结果表明，VP-TDH的第33 ~ 100位氨基酸残基对VP-TDH的热稳定性具有重要作用; 6）这些嵌合毒素还表明第89和91位氨基酸残基对VP-TDH的红细胞特异性具有重要作用; 7）VP-TDH的作用方式分析表明，VP-TDH作为一种“预形成毒素“发挥作用; 8）VP-TDH与VP-TDH之间的相互作用表明，VP-TDH的热稳定性与VP-TDH的热稳定性密切相关对副溶血性弧菌产生的皮利进行了纯化和鉴定。9）副溶血性弧菌的血凝素被证明是该微生物可能的定殖因子，

In this study, we investigated the pathogenic mechanism of gastroenteritis due to Vibrio parahaemolyticus. Following results were obtained through this experiment.1) A Vp-TDH-related hemolysin (named VP-TRH) was discovered as a virulence factor from human isolates of Kanagawa phenoffienon-negative V. parahaemolyticus2) Production of Vp-TDH/TRH-related toxins by V. choleras non-51, V. mimicus, and V. hollisae was demonstrated. These VP-TDH/TRH family toxins were purified3) and iharacterized. Gene cloning of these toxins was also performed.3) Immunological properties of VP-TDH/TRH were analysed by both monoclonal and polyclonal antibodies.4) Importance of amino acid residues at 60 and 90 position from the N terminus of VP-TDH for. expressing biological. activity was-demonstrated.5) By preparing chimeric toxins, importance of amino acid residues from 33 to 100 for the heat-stability of Vp-TDH was demonstrated.6) These chimeric toxins also suggested the importance of two amino acid residues at 89 and 91 for the erythrocyte-specificity.7) Analysis of mode of action of VP-TDH revealed that VP-TDH acts as a "preforming toxin"8) Purification and characterization of pili produced by V. parahaemolytiucs were performed.9) Haemagglutinins of V. parahaemolyticus was demonstrated to be a possible colonization factor of this organism,