权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

Study on the early process (especially adhesive and invasive process) of pathogenesis of bacterial enteropathogens and its cellular response

细菌性肠病菌发病的早期过程（特别是粘附和侵袭过程）及其细胞反应研究

基本信息

批准号：
14021055
负责人：
HONDA Takeshi
金额：
$ 38.4万
依托单位：
Osaka University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research on Priority Areas
财政年份：
2002
资助国家：
日本
起止时间：
2002 至 2005
项目状态：
已结题

项目摘要

(1) Result on enteropathogenic E. coli (EPEC, EHEC) : We found that cortactin and talin bind to Tir, an effector injected vir Type III secretion system (TTSS) and the binding is essential for causing attaching and effacing lesion formed by EPEC and EHEC. We also found that EspB forming tip of needle of TTSS also bind host molecule of a catenin which is essential for the A/E lesion formation.(2) Result on Vibrio parahaemolyticus : The most important virulence factor of this organism is TDH../TRH (an exotoxin), however, this may not be enough to full understand of pathogenesis. Whole genome analysis completed suggested us that this has two sets of TTSSs. TTSS-1 is present in large chromosome and secrete an effector having apoptotic cytotoxicity. On the other hand, TTSS-2 found in only clinical isolates is locate on small chromosome and unidentified effector which may have enteropathogenicity may be imvolved.(3) Result on Providencia alcalifaciens : We have found, for the first time, an outbreak attributed to P alcalifaciens (PA). We also found that this organism possess TTSS on a large plasmid.. As shown above and unlike our speculation that these 3 pathogens may have different mechanism for causing diseases, it became clear that the 3 pathogens possess a common process, TTSS, which may serve as one of the important virulence factor.

(1)肠源性大肠埃希菌(EPEC，EHEC)的研究结果：我们发现Cortactin和talin与TIR结合，而TTSS是一种注射VIR的效应器，这种结合是导致EPEC和EHEC形成附着和消除病变所必需的。我们还发现，TTSS的针尖形成EspB还结合了A/E病变形成所必需的连接素的宿主分子。(2)对副溶血性弧菌的研究结果：该菌最重要的毒力因子是TDH./TRH(一种外毒素)，但这可能不足以充分了解其致病机理。完成的全基因组分析表明，这有两套TTSS。TTSS-1存在于大染色体中，分泌一种具有细胞毒作用的效应分子。另一方面，仅在临床分离株中发现的TTSS-2位于小染色体上，可能存在可能具有肠道致病性的不明效应物。(3)对普罗维登西亚致病作用的研究结果：我们首次发现了一起由阿卡利伯氏菌(PA)引起的暴发。我们还发现，该菌在一个大的质粒上具有TTSS。如上所述，与我们推测这3种病原体可能具有不同的致病机制不同，很明显，这3种病原体具有共同的过程TTSS，这可能是重要的毒力因子之一。