Study on molecules which exhibit either inhibitory or stimulating activities to growth of pathogen, especially Vibribparahaemolyticus

对病原体特别是副溶血弧菌生长具有抑制或刺激活性的分子的研究

• 批准号: 18390135
• 负责人: HONDA Takeshi
• 金额: $ 10.77万
• 依托单位: Osaka University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2006
• 资助国家: 日本
• 起止时间: 2006 至 2007
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-18390135/
• 关键词: Serum; growth factor; inhibitor; Vibiro narahaemolvticus; transferin; hemonexin

We found the activities which inhibit or stimulate the growth of bacteria in bovine serum and human serum as well. Inhibitory molecule for the bacterial growth (especially Vibrio parahaemolyticus) was purified by successive column chromatographies of Sephadex G75 and DEAE-Sephadex G25 and Mono Q (FPLC), and we obtained the molecules of either transferin or hemopexin or both. On the other hand, we are close to obtain highly purified molecule which exhibits stimulating activity to bacterial growth. The molecular weight seems to be less than 3000, and it is heat-stable (at 100℃). It contains Mg+ and Mo+. In conclusion, we found that there are still unidentified factors in sera which exhibit either inhibitory on stimulating activities on growth of pathogen.

我们还发现了牛血清和人血清中抑制或刺激细菌生长的活性。用Sephadex G75、DEAE-Sephadex G25和Mono Q(FPLC)柱层析纯化了抑制细菌生长的分子(尤其是副溶血性弧菌)，得到了转铁蛋白和/或血凝素分子。另一方面，我们即将获得对细菌生长具有刺激活性的高纯度分子。其相对分子质量似乎小于3,000，并且具有热稳定性(100℃)。它含有Mg+和Mo+。综上所述，我们发现血清中仍有一些未知因素对病原菌的生长有抑制作用。

项目成果

細菌性腸管感染症研究の現状

细菌性肠道感染研究现状

• 发表时间: 2007
• 作者: 韓賢子;蒲池一成;桑江朝臣;阿部章夫;荒川宜親;本田武司
• 通讯作者: 本田武司

Cortactin is essential for F-actin assembly in enteropathogenic Escherichia coli(EHEC)-and enterohaemorrhagic E. coli(EHEC)-induced pedestals and the α-helical region is involved in the localization of cortactin to bacterial attachment sites.

Cortactin 对于肠致病性大肠杆菌 (EHEC) 和肠出血性大肠杆菌 (EHEC) 诱导的基座中的 F-肌动蛋白组装至关重要，并且 α-螺旋区域参与 Cortactin 定位到细菌附着位点。

• 发表时间: 2006
• 期刊: Cellular Microbiology 8(5)
• 作者: Vlademir. C.;T. Kodama;N. Nijjstad;S. K. Abolghait;T. Iida and T. Honda.
• 通讯作者: T. Iida and T. Honda.

腸炎ビブリオ食中毒の正しい知識をもつ 食中毒検査・診断のコッと落とし穴(渡辺治雄編)

副溶血性弧菌食物中毒的准确知识：食物中毒检测和诊断的技巧和陷阱（渡边春夫编辑）

• 发表时间: 2006
• 作者: 朴権三;本田武司(分担)
• 通讯作者: 本田武司(分担)

Two type IV pili of Vibrio parahaemolyticus play different roles in biofilm formation

• DOI: 10.1111/j.1574-6968.2006.00438.x
• 发表时间: 2006-11-01
• 期刊: FEMS MICROBIOLOGY LETTERS
• 影响因子: 2.1
• 作者: Shime-Hattori, Akiko;Iida, Tetsuya;Honda, Takeshi
• 通讯作者: Honda, Takeshi

Identification of proteins secreted via Vibrio parahaemolyticus Typeffisecretion system 1.

副溶血性弧菌分泌系统分泌的蛋白质的鉴定1。

• 发表时间: 2006
• 期刊: Infect. Immun 74(2)
• 作者: Ono.;T.;K.S.;Park;M.;Ueta;T.;lida;T.;Honda
• 通讯作者: Honda