Molecular biochemical study on pathogenesis, especially toxin and adhesive factor, of Vibrio parahaemolyticus

副溶血性弧菌发病机制尤其是毒素和粘附因子的分子生化研究

• 批准号: 07407008
• 负责人: HONDA Takeshi
• 金额: $ 11.01万
• 依托单位: Osaka University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (A)
• 财政年份: 1995
• 资助国家: 日本
• 起止时间: 1995 至 1997
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07407008/
• 关键词: Thermostable direct hemolysin(TDH); TDH-related hemolysin (TRH); Vibrio parahaemolytiucs; Adhesion; 細胞浸入性; 定着因子; レセプター; リン酸化; 毒素; 変異毒素; サイトトキシン

The purpose of this research is to investigate detailed pathogenesis of Vibrio parahaemolyticus. Following results were obtained.1. Studies on thermostable direct hemolysin (TDH) and TDH-related hemolysin (TRH) : We found that TDH recognized a specific receptor through its N-teminal region and then induced phosphorylation of a 25 kDa protein, resulting in hemolysis. We also found that genes for TDH,TRH and urease are closely linked and present within 50kb-fragment (a pathogenicity island? ).2. Studies on adhesive factors : We identified cHA (cell-associated hemagglutinin) as an adhesive factor of V.parahaemolyticus. The cHA is a tetrameric protein consisting of 4 subunit of 26kDa and the receptor for cHA is a glycoconjugate having molecular weight of 17kDa.3. Other results : We also demonstrated the invasive character of V.parahaemolyticus in vitro cultured cell system, which may explain the cause of septicemia due to this organism. The methods detecting TDH,TRH or both were developed and evaluated, and a new variant of TDH was found.

本研究的目的是调查副溶血弧菌的详细发病机制。得到如下结果： 1．热稳定性直接溶血素（TDH）和TDH相关溶血素（TRH）的研究：我们发现TDH通过其N末端区域识别特定受体，然后诱导25 kDa蛋白质磷酸化，导致溶血。我们还发现TDH、TRH和脲酶基因紧密相连并存在于50kb片段内（致病岛？）。2．粘附因子的研究：我们鉴定出 cHA（细胞相关血凝素）是副溶血弧菌的粘附因子。 cHA是由4个26kDa的亚基组成的四聚体蛋白质，cHA的受体是分子量为17kDa的糖缀合物。3。其他结果：我们还在体外培养细胞系统中证明了副溶血弧菌的侵袭特性，这可能解释了该生物体引起败血症的原因。开发并评估了检测TDH、TRH或两者的方法，并发现了TDH的新变体。

项目成果

本田 武司: "感染症の現状と展望(細菌感染症)" 臨床科学. 33. 1038-1042 (1997)

Takeshi Honda：“传染病（细菌感染）的现状和前景”《临床科学》33。1038-1042（1997）。

Suthienkul O., T.Iida, K.S.Park, M.Ishibashi, S.Supavej, K.Yamamoto and T.Honda: "Restriction fragment length polymorphism of the tdh and trh genes in clinical Vibrio parahaemolyticus strains." J.Clin.Microbiol.34 (5). 1293-1295 (1996)

Suthienkul O.、T.Iida、K.S.Park、M.Ishibashi、S.Supavej、K.Yamamoto 和 T.Honda：“临床副溶血性弧菌菌株中 tdh 和 trh 基因的限制性片段长度多态性。”

本田武司、上田成子(共著): "「食中毒予防と対処のすべて」。" 法研, 212 (1997)

本田武 (Takeshi Honda) 和上田鸣子 (Naruko Ueda)（合著者）：“有关预防和处理食物中毒的一切。”

Nagamune,K.et al.: "Intramolecular chaperone activity of the pro-region of Vibrio cholerae El Tor cytolyisn." J.Biological Chem.272(2). 1338-1343 (1997)

Nagamune,K.et al.：“霍乱弧菌 El Tor 溶细胞作用前区的分子内伴侣活性。”

山本耕一郎他: "モダンメディア" 細菌性腸感染症に対するワクチン現状, 42(9):394-399 (1996)

Koichiro Yamamoto 等人：《现代媒体》细菌性肠道感染疫苗的现状，42(9):394-399 (1996)