Study on Non-Invasive Assessment of Multiple Organ Function with In vivo ^<31>P-MRS

体内^<31>P-MRS无创评估多器官功能的研究

• 批准号: 01480341
• 负责人: HIROSE Hajime
• 金额: $ 1.28万
• 依托单位: Gifu University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (B)
• 财政年份: 1989
• 资助国家: 日本
• 起止时间: 1989 至 1990
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-01480341/
• 关键词: Organ transplantation; Organ preservation; Non-invasive assessment of multiple organ function; Organ perfusion; High energy phosphate; ^<31>P-MRS; ^<31>PーMRS; ^<31>P-MRS

Using a whole body MRI device, we investigated functional evaluation method of a graft in vivo. First of all, phosphorus compounds in perfused pig livers were measured with ^<31>P-MRS. In this experiments, only inorganic phosphate, PME, PDE and NADH could be observed, because perfusion was not sufficient. Then, we observed phosphorus compounds during perfusion by artificial heart-lung machine in livers isolated from mongrel dogs. In this experiment, high-energy phosphates (HEP) could be observed, and it was suggested that HEP levels in organs are affected by perfusion flow.In the second experiment, in vivo ^<31>P-MRS was applied for human livers, after designation of regions of interest (DRESS method). In this experiment specific spectrum of the liver were observed. Following this finding, we tried in vivo ^<31>P-MRS in patients before liver operation and found different spectra between cirrhotic liver and cancer-affected livers. This result indicates that the in vivo ^<31>P-MRS is use … More ful method for functional evaluation of the donor liver before harvesting.Using an experimental NMR device (6.4T), we obtained several basic data for evaluation of in vivo MRS. Changes of spectrum in NMR depending on temperatures were studied in isolated rat hearts for cardiac viability during cold storage. As a result, the spectra obtained at different temperatures could be compared to each other if they are corrected for the influence from changes in T_1 time and probe sensitivity. Following this finding, we assessed the University of Wisconsin solution on isolated rat hearts and found its usefulness. Furthermore, we studied metabolism of phosphates during warm ischemia of perfused livers, and compared the HEP determined by a biochemical method (HPLC) and the HEP determined by NMR.And, we studied tissue blood flow, pressure volume curves with a conductance catheter and electromicroscopic pathologic assessment in heterotopic dog heart transplantation model. These data may applied for basic data of in vivo MRS measurements. Less

利用全身磁共振成像设备，我们研究了在体移植物的功能评价方法。本实验首先用~ 1H-P-MRS测定了灌注猪肝中的磷化合物<31>，由于灌注不充分，只能观察到无机磷酸盐、PME、PDE和NADH。然后，我们观察了人工心肺机灌注过程中的磷化合物从杂种狗的肝脏分离。本实验中，我们观察到了高能磷酸盐（HEP）的存在，并认为灌注流量对器官中HEP的水平有影响。第二个实验中，<31>我们对人体肝脏进行了在体1HP-MRS，并对肝脏进行了感兴趣区域的设计（DRESS法）。在本实验中，观察到肝脏的特定光谱。根据这一发现，我们在<31>肝脏手术前对患者进行了体内13 P-MRS试验，发现肝硬化和癌症影响的肝脏之间存在不同的谱。这一结果表明，在体内13 <31>P-MRS是使用 ...更多信息 本文介绍了一种新的活体供肝功能评价方法，利用6.4T核磁共振装置，获得了活体供肝MRS的基本数据，并研究了冷保存条件下离体大鼠心脏的NMR谱随温度的变化。结果表明，如果对T_1时间和探针灵敏度的影响进行校正，不同温度下获得的光谱可以相互比较。根据这一发现，我们评估了威斯康星州大学的解决方案在离体大鼠心脏，并发现其有用性。此外，我们还研究了热缺血时肝脏磷酸盐的代谢，比较了用生化方法（HPLC）和核磁共振（NMR）测定的HEP，并研究了异位犬心脏移植模型的组织血流量、压力-容积曲线和电镜病理学评价。这些数据可作为在体MRS测量的基础数据。少

项目成果

松波 英寿,鬼束惇義,広瀬 一: "MRSによる術前肝機能評価" モダンメディシン. 18. 66-69 (1989)

Hidetoshi Matsunami、Atsuyoshi Onizuka、Hajime Hirose：“术前 MRS 肝功能评估”现代医学 18. 66-69 (1989)。

Y Mori, S Murakawa, H Sasaki, T Yamada, K Azuma, M Kokubo, S Umeda, H Hirose :"Peripheral tissue blood flow measured by Laser flow meter during extracorporeal circulation in open heart surgery." The Journal of Japanese Society for Laser Medicine. 10(3) :.

Y Mori、S Murakawa、H Sasaki、T Yamada、K Azuma、M Kokubo、S Umeda、H Hirose：“在心脏直视手术中体外循环期间用激光流量计测量外周组织血流量。”

H MAtsunami,H Hirose,A Onitsuka,SV Lynch,GA Balderson,RW Strong: "^<31>PーMagnetic Resonance SpECtroscopy in Evaluating Hepatic Function:A Possible Application in Donor Assessment Prior to Liver Transplantation." Transplantation Proceedings. 22. 2146-2148

H MAtsunami、H Hirose、A Onitsuka、SV Lynch、GA Balderson、RW Strong：“^<31>P 磁共振波谱镜在评估肝功能中的应用：在肝移植手术前的供体评估中的可能应用。” 2146-2148

H Matsunami, H Hirose, A Onitsuka, SV Lynch, GA Balderson, RW Strong :" ^<31>P-Magnetic resonance spectroscopy in evaluating hepatic function : A possible application in donor assessment prior to liver transplantation." Transplantation Proceeding. 22. 214

H Matsunami、H Hirose、A Onitsuka、SV Lynch、GA Balderson、RW Strong：“^ 31 P-磁共振波谱法在评估肝功能中的应用：肝移植前供体评估的可能应用。”

森 義雄、村川 真司、佐々木 裕茂、山田 拓,東 健一郎,小久保 光治、梅田 正五、広瀬 一: "レザ-ドップラ-血流量計による 体外循環中末梢組織血流量に関する検討。" レ-ザ-医学会雑誌,. 10. 485-488 (1989)

Yoshio Mori、Shinji Murakawa、Hiroshige Sasaki、Taku Yamada、Kenichiro Higashi、Mitsuharu Kokubo、Shogo Umeda、Hajime Hirose：“使用激光多普勒流量计研究体外循环期间的外周组织血流，10。485-” 488 (1989)