Establishment of NOS-gene transferred endothelial cells coated vascular prostheses

NOS基因转染内皮细胞涂层人工血管的建立

• 批准号: 10671246
• 负责人: HIROSE Hajime
• 金额: $ 2.24万
• 依托单位: Gifu University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1998
• 资助国家: 日本
• 起止时间: 1998 至 1999
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-10671246/
• 关键词: gene transfer; gene gun; vascular prostheses; ハイブリッド型人工血管; Nitric Oxide synthase(NOS); リポソーム

It is an important issue to regulate the occlusion of small sized vascular prostheses, that achieve constitutive patency even in coronary artery bypass grafting (CABG). For this issue, we designed NOS-gene transferred endotherial cells coated vascular prostheses. NOS-gene transferred endotherial cells will prevent thrombopoietic occlusion or vascular stenosis associated with the endotherial proliferation.We investigated how the endotherial cell proliferation caused the occlusion using peripheral progenitor endotherial cells. Progenitor endotherial cells were obtained from human peripheral blood using the anti-CD34 antibody-coated magnet beads method. We observed that progenitor endotherial cells developed to endotherial cells in the culture with bovine brain extract, which was endotherial growth factor. Conversely, we established gene transfer system using gene gun, that achieved high gene expression.Therefore, we have the next issue to apply progenitor endotherial cells onto the vascular prostheses. In addition, we are going to investigate whether or not NOS gene transfer using gene gun is useful to prevent the endotherial cell proliferation in small sized vascular prostheses.

小尺寸人造血管的闭塞调节是一个重要的问题，即使在冠状动脉旁路移植术（CABG）中也能实现结构性通畅。针对这一问题，我们设计了NOS基因转染内皮细胞涂层人工血管。一氧化氮合酶基因转染的内皮细胞可以预防与内皮细胞增殖相关的血栓形成性闭塞或血管狭窄，本实验利用外周血内皮祖细胞研究了内皮细胞增殖是如何引起血栓形成性闭塞的。使用抗CD34抗体包被磁珠法从人外周血获得祖内皮细胞。我们观察到内皮祖细胞在含有内皮生长因子的牛脑提取物的培养液中发育为内皮细胞。相反，我们建立了基因枪转基因系统，实现了基因的高表达，因此，我们的下一个问题是将内皮祖细胞应用于人造血管。此外，我们还将研究基因枪介导的NOS基因转染是否有助于防止小尺寸人工血管中内皮细胞的增殖。

项目成果

Tadamasa Miyauchi, Hajime Hirose, Eisaku Sasaki, Masatoshi Hayashi, Yoshio Mori, Shinji Murakawa, Hisato Takagi, Hiroyuki Yasuda, Yoshitaka Kumada, Hisashi Iwata: "Predictability of Dielectric Properties for Ischemic Injury of the Skeletal Muscle before R

Tadamasa Miyauchi、Hajime Hirose、Eisaku Sasaki、Masatoshi Hayashi、Yoshio Mori、Shinji Murakawa、Hisato Takagi、Hiroyuki Yasuda、Yoshitaka Kumada、Hisashi Iwata：“R 前骨骼肌缺血性损伤介电特性的预测