Establishment of NOS-gene transferred endothelial cells coated vascular prostheses

即使在冠状动脉旁路移植术（CABG）中，调节小血管假体的闭塞也是一个重要的问题。为此，我们设计了nos基因转移内皮细胞包被血管假体。nos基因转移的内皮细胞可防止与内皮增生相关的血栓形成阻塞或血管狭窄。我们利用外周祖内皮细胞研究内皮细胞增殖是如何引起闭塞的。采用抗cd34抗体包被磁珠法从人外周血中获得祖细胞内皮细胞。我们观察到，牛脑提取物作为内皮生长因子，在培养过程中，祖内皮细胞向内皮细胞发展。反过来，我们利用基因枪建立了基因转移系统，实现了基因的高表达。因此，将祖内皮细胞应用于血管假体是下一步的研究方向。此外，我们还将研究利用基因枪转移NOS基因是否有助于阻止小血管假体内皮细胞的增殖。

It is an important issue to regulate the occlusion of small sized vascular prostheses, that achieve constitutive patency even in coronary artery bypass grafting (CABG). For this issue, we designed NOS-gene transferred endotherial cells coated vascular prostheses. NOS-gene transferred endotherial cells will prevent thrombopoietic occlusion or vascular stenosis associated with the endotherial proliferation.We investigated how the endotherial cell proliferation caused the occlusion using peripheral progenitor endotherial cells. Progenitor endotherial cells were obtained from human peripheral blood using the anti-CD34 antibody-coated magnet beads method. We observed that progenitor endotherial cells developed to endotherial cells in the culture with bovine brain extract, which was endotherial growth factor. Conversely, we established gene transfer system using gene gun, that achieved high gene expression.Therefore, we have the next issue to apply progenitor endotherial cells onto the vascular prostheses. In addition, we are going to investigate whether or not NOS gene transfer using gene gun is useful to prevent the endotherial cell proliferation in small sized vascular prostheses.