Gene Therapy for Vein Grafts Stenosis after Coronary Artery Bypass Grafting.

冠状动脉搭桥术后静脉移植物狭窄的基因治疗。

• 批准号: 09557108
• 负责人: HIROSE Hajime
• 金额: $ 7.55万
• 依托单位: Gifu University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 1997
• 资助国家: 日本
• 起止时间: 1997 至 1998
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09557108/
• 关键词: endothelial cell; coronary artery bypass grafting; C-type natriuretic peptide; CD34 positive progenitor endothelial cell; C-type natriuretic peptide; 遺伝子治療; リポソーム法; グラフト内膜肥厚

It is an important issue to regulate the occlusion. of vein grafts after coronary artery bypass grafting (CABG) for ischemic heart disease. We investigated how the endothelial cell proliferation in vein grafts caused the occlusion of vein grafts using peripheral progenitor endothelial cells. Progenitor endothelial cells were obtained from human peripheral blood using the anti-CD34 antibody-coated magnet beads method. We observed that progenitor endothelial cells developed to endothelial cells in the culture with bovine brain extract, which was endothelial growth factor.Conversely, we measured c-type natriuretic peptide (CNP), which inhibited endothelial cell proliferation as well as one of urine progressing peptide family, In the patients for CABG, CNP increased only during extracorponeal circulation. When we utilize constitutive express CNP, there are possibility to prevent the occlusion of vein grafts.Therefore, We have the next issue to analyze the relationship between progenitor endothelial cells and CNP.In addition, we are going to investigate whether or not CNP gene therapy will be useful to prevent the endothelial cell proliferation in venus graft.

对咬合的调节是一个重要的问题。缺血性心脏病冠状动脉旁路移植术（CABG）后静脉移植的研究我们利用外周祖内皮细胞研究了内皮细胞在静脉移植物中的增殖是如何引起静脉移植物闭塞的。采用抗cd34抗体包被磁珠法从人外周血中获得祖内皮细胞。我们观察到祖内皮细胞在牛脑提取物（内皮生长因子）的培养下发育成内皮细胞。相反，我们测量了c型利钠肽（CNP），它抑制内皮细胞增殖以及尿进展肽家族之一，在CABG患者中，CNP仅在体外循环中增加。当我们使用本构表达CNP时，有可能防止移植物静脉闭塞。因此，我们的下一个问题是分析祖内皮细胞与CNP的关系。此外，我们将探讨CNP基因治疗是否有助于防止血管内皮细胞的增殖。

项目成果

今泉 松久: "動脈硬化性変化からみた弓部大動脈分枝の形態学的検討脳分離体外循環における送血部位選択に関して" 岐阜大学医学部紀要. 46. 146-151 (1998)

今泉松久：“从动脉硬化变化的角度进行主动脉弓分支的形态学研究。关于离体脑体外循环中血液输送部位的选择”岐阜大学医学院通报46。146-151（1998）。

Matsuhisa IMAIZUMI: "Morphological Analysis on Atherosclerotic Changes in Branches from the Aortic Arch On the Sites of Arterial Cannulation for Selective Cerebral Perfusion." ACTA SCHOLAE MEDICINALIS UNIVERSITATIS IN GIFU. Vol.46 No.2. 146-151 (1998)

Matsuhisa IMAIZUMI：“选择性脑灌注动脉插管部位主动脉弓分支动脉粥样硬化变化的形态学分析”。