Gene Therapy for Vein Grafts Stenosis after Coronary Artery Bypass Grafting.

咬合调节是一个重要的问题。冠状动脉旁路移植术（CABG）后静脉移植物的缺血性心脏病。我们使用外周祖内皮细胞研究了移植静脉中内皮细胞增殖是如何引起移植静脉闭塞的。使用抗CD 34抗体包被磁珠法从人外周血获得祖内皮细胞。我们观察到内皮祖细胞在牛脑提取物（endothelial growth factor，内皮生长因子）的作用下向内皮细胞分化，同时测定了C型利钠肽（c-type natriuretic peptide，CNP）的含量。当我们利用组成型表达CNP时，有可能防止静脉移植物的闭塞，因此，我们的下一个问题是分析内皮祖细胞与CNP的关系，此外，我们将研究CNP基因治疗是否有助于防止静脉移植物中的内皮细胞增殖。

It is an important issue to regulate the occlusion. of vein grafts after coronary artery bypass grafting (CABG) for ischemic heart disease. We investigated how the endothelial cell proliferation in vein grafts caused the occlusion of vein grafts using peripheral progenitor endothelial cells. Progenitor endothelial cells were obtained from human peripheral blood using the anti-CD34 antibody-coated magnet beads method. We observed that progenitor endothelial cells developed to endothelial cells in the culture with bovine brain extract, which was endothelial growth factor.Conversely, we measured c-type natriuretic peptide (CNP), which inhibited endothelial cell proliferation as well as one of urine progressing peptide family, In the patients for CABG, CNP increased only during extracorponeal circulation. When we utilize constitutive express CNP, there are possibility to prevent the occlusion of vein grafts.Therefore, We have the next issue to analyze the relationship between progenitor endothelial cells and CNP.In addition, we are going to investigate whether or not CNP gene therapy will be useful to prevent the endothelial cell proliferation in venus graft.